Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins

Oren Parnas; Rona Amishay; Batia Liefshitz; Adi Zipin-Roitman; Martin Kupiec

doi:10.4161/cc.10.17.16778

Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins

Oren Parnas, Rona Amishay, Batia Liefshitz, Adi Zipin-Roitman, Martin Kupiec^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

PCNA is a homotrimeric ring with important roles in DNA replication and repair. PCNA is loaded and unloaded by the RFC complex, which is composed of five subunits (Rfc1-5). Three additional complexes that share with RFC the small subunits (Rfc2-5) and contain alternative large subunits were found in yeast and other eukaryotes. We have recently reported that one of these, the Elg1-RFC complex, interacts with SUMOylated PCNA and may play a role in its unloading during DNA repair. Here we report that a yeast-two-hybrid screen with the N terminus of Elg1(which interacts with SUMOylated PCNA) uncovered interactions with proteins that belong to the SUMO pathway, including Slx5 and Slx8, which form an E3 ubiquitin ligase that ubiquitinates SUMOylated proteins. Mutations in SLX5 result in a genomic instability phenotype similar to that of elg1 mutants. The physical interaction between the N terminus of Elg1 and Slx5 is mediated by poly-SUMO chains but not by PCNA modifications, and requires Siz2, but not Siz1, activity. Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent.

Original language	English
Pages (from-to)	2894-2903
Number of pages	10
Journal	Cell Cycle
Volume	10
Issue number	17
DOIs	https://doi.org/10.4161/cc.10.17.16778
State	Published - 1 Sep 2011
Externally published	Yes

Bibliographical note

Funding Information:
stringent conditions). Plasmids were isolated from positive clones We thank Stefan Jentsch, Helle Ulrich, Jurgen Dohmen, Steven and re-transformed to validate results. J. Brill, Mark Hochstrasser, Daniel Durocher, Erica S. Johnson, DNA damage sensitivity. To test chronic exposure to DNA Philip Hieter and Peter Burgers for reagents. We thank all the damaging agents, serial 10-fold dilutions of logarithmic yeast members of the Kupiec lab for advice and support. M.K. was sup-cells were spotted on fresh SD-complete plates with or without ported by grants from the Israel Cancer Research Fund and the MMS (Sigma), and incubated at 30°C for three days. Israel Cancer Association. Recombination/Mutation assays. The rate of recombination was measured in derivatives of strain MK166, as described in reference 50. This strain allows one to measure forward mutation at CAN1 (white Canavanine-resistant colonies), recombination

Keywords

PCNA
STUbL
SUMO
Yeast
elg1
slx5-slx8

Access to Document

10.4161/cc.10.17.16778

Cite this

@article{b72167de63b94f16ac86dacd10ff9e1c,

title = "Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins",

abstract = "PCNA is a homotrimeric ring with important roles in DNA replication and repair. PCNA is loaded and unloaded by the RFC complex, which is composed of five subunits (Rfc1-5). Three additional complexes that share with RFC the small subunits (Rfc2-5) and contain alternative large subunits were found in yeast and other eukaryotes. We have recently reported that one of these, the Elg1-RFC complex, interacts with SUMOylated PCNA and may play a role in its unloading during DNA repair. Here we report that a yeast-two-hybrid screen with the N terminus of Elg1(which interacts with SUMOylated PCNA) uncovered interactions with proteins that belong to the SUMO pathway, including Slx5 and Slx8, which form an E3 ubiquitin ligase that ubiquitinates SUMOylated proteins. Mutations in SLX5 result in a genomic instability phenotype similar to that of elg1 mutants. The physical interaction between the N terminus of Elg1 and Slx5 is mediated by poly-SUMO chains but not by PCNA modifications, and requires Siz2, but not Siz1, activity. Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent.",

keywords = "PCNA, STUbL, SUMO, Yeast, elg1, slx5-slx8",

author = "Oren Parnas and Rona Amishay and Batia Liefshitz and Adi Zipin-Roitman and Martin Kupiec",

note = "Funding Information: stringent conditions). Plasmids were isolated from positive clones We thank Stefan Jentsch, Helle Ulrich, Jurgen Dohmen, Steven and re-transformed to validate results. J. Brill, Mark Hochstrasser, Daniel Durocher, Erica S. Johnson, DNA damage sensitivity. To test chronic exposure to DNA Philip Hieter and Peter Burgers for reagents. We thank all the damaging agents, serial 10-fold dilutions of logarithmic yeast members of the Kupiec lab for advice and support. M.K. was sup-cells were spotted on fresh SD-complete plates with or without ported by grants from the Israel Cancer Research Fund and the MMS (Sigma), and incubated at 30°C for three days. Israel Cancer Association. Recombination/Mutation assays. The rate of recombination was measured in derivatives of strain MK166, as described in reference 50. This strain allows one to measure forward mutation at CAN1 (white Canavanine-resistant colonies), recombination",

year = "2011",

month = sep,

day = "1",

doi = "10.4161/cc.10.17.16778",

language = "אנגלית",

volume = "10",

pages = "2894--2903",

journal = "Cell Cycle",

issn = "1538-4101",

publisher = "Taylor and Francis Ltd.",

number = "17",

}

TY - JOUR

T1 - Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins

AU - Parnas, Oren

AU - Amishay, Rona

AU - Liefshitz, Batia

AU - Zipin-Roitman, Adi

AU - Kupiec, Martin

N1 - Funding Information: stringent conditions). Plasmids were isolated from positive clones We thank Stefan Jentsch, Helle Ulrich, Jurgen Dohmen, Steven and re-transformed to validate results. J. Brill, Mark Hochstrasser, Daniel Durocher, Erica S. Johnson, DNA damage sensitivity. To test chronic exposure to DNA Philip Hieter and Peter Burgers for reagents. We thank all the damaging agents, serial 10-fold dilutions of logarithmic yeast members of the Kupiec lab for advice and support. M.K. was sup-cells were spotted on fresh SD-complete plates with or without ported by grants from the Israel Cancer Research Fund and the MMS (Sigma), and incubated at 30°C for three days. Israel Cancer Association. Recombination/Mutation assays. The rate of recombination was measured in derivatives of strain MK166, as described in reference 50. This strain allows one to measure forward mutation at CAN1 (white Canavanine-resistant colonies), recombination

PY - 2011/9/1

Y1 - 2011/9/1

N2 - PCNA is a homotrimeric ring with important roles in DNA replication and repair. PCNA is loaded and unloaded by the RFC complex, which is composed of five subunits (Rfc1-5). Three additional complexes that share with RFC the small subunits (Rfc2-5) and contain alternative large subunits were found in yeast and other eukaryotes. We have recently reported that one of these, the Elg1-RFC complex, interacts with SUMOylated PCNA and may play a role in its unloading during DNA repair. Here we report that a yeast-two-hybrid screen with the N terminus of Elg1(which interacts with SUMOylated PCNA) uncovered interactions with proteins that belong to the SUMO pathway, including Slx5 and Slx8, which form an E3 ubiquitin ligase that ubiquitinates SUMOylated proteins. Mutations in SLX5 result in a genomic instability phenotype similar to that of elg1 mutants. The physical interaction between the N terminus of Elg1 and Slx5 is mediated by poly-SUMO chains but not by PCNA modifications, and requires Siz2, but not Siz1, activity. Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent.

AB - PCNA is a homotrimeric ring with important roles in DNA replication and repair. PCNA is loaded and unloaded by the RFC complex, which is composed of five subunits (Rfc1-5). Three additional complexes that share with RFC the small subunits (Rfc2-5) and contain alternative large subunits were found in yeast and other eukaryotes. We have recently reported that one of these, the Elg1-RFC complex, interacts with SUMOylated PCNA and may play a role in its unloading during DNA repair. Here we report that a yeast-two-hybrid screen with the N terminus of Elg1(which interacts with SUMOylated PCNA) uncovered interactions with proteins that belong to the SUMO pathway, including Slx5 and Slx8, which form an E3 ubiquitin ligase that ubiquitinates SUMOylated proteins. Mutations in SLX5 result in a genomic instability phenotype similar to that of elg1 mutants. The physical interaction between the N terminus of Elg1 and Slx5 is mediated by poly-SUMO chains but not by PCNA modifications, and requires Siz2, but not Siz1, activity. Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent.

KW - PCNA

KW - STUbL

KW - SUMO

KW - Yeast

KW - elg1

KW - slx5-slx8

UR - http://www.scopus.com/inward/record.url?scp=80052425790&partnerID=8YFLogxK

U2 - 10.4161/cc.10.17.16778

DO - 10.4161/cc.10.17.16778

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:80052425790

SN - 1538-4101

VL - 10

SP - 2894

EP - 2903

JO - Cell Cycle

JF - Cell Cycle

IS - 17

ER -

Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this