Abstract
Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.
Original language | English |
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Pages (from-to) | 145-149 |
Number of pages | 5 |
Journal | FEMS Microbiology Letters |
Volume | 50 |
Issue number | 2-3 |
DOIs | |
State | Published - May 1988 |
Externally published | Yes |
Bibliographical note
Funding Information:This study was supportedb y a grant from the United States-IsraeBl inationalAgricultural Re-searcha nd DevelopmenFtu nd (BARD). We thank S. Levisohnf or the M. gallisepticumst rains,G .E. Kenny for the M. pneumoniaset rains,N . Brot for providingt he tufA plasmid,a nd M. Wormserf or valuablet echnicaal ssistance.
Keywords
- Elongation factor gene
- Genetic polymorphism
- Mycoplasma