Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains

D. Yogev*, S. Sela, H. Bercovier, S. Razin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.

Original languageAmerican English
Pages (from-to)145-149
Number of pages5
JournalFEMS Microbiology Letters
Volume50
Issue number2-3
DOIs
StatePublished - May 1988
Externally publishedYes

Bibliographical note

Funding Information:
This study was supportedb y a grant from the United States-IsraeBl inationalAgricultural Re-searcha nd DevelopmenFtu nd (BARD). We thank S. Levisohnf or the M. gallisepticumst rains,G .E. Kenny for the M. pneumoniaset rains,N . Brot for providingt he tufA plasmid,a nd M. Wormserf or valuablet echnicaal ssistance.

Keywords

  • Elongation factor gene
  • Genetic polymorphism
  • Mycoplasma

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