Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains

D. Yogev; S. Sela; H. Bercovier; S. Razin

doi:10.1111/j.1574-6968.1988.tb02928.x

Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains

D. Yogev^*, S. Sela, H. Bercovier, S. Razin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.

Original language	English
Pages (from-to)	145-149
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	50
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1988.tb02928.x
State	Published - May 1988
Externally published	Yes

Bibliographical note

Funding Information:
This study was supportedb y a grant from the United States-IsraeBl inationalAgricultural Re-searcha nd DevelopmenFtu nd (BARD). We thank S. Levisohnf or the M. gallisepticumst rains,G .E. Kenny for the M. pneumoniaset rains,N . Brot for providingt he tufA plasmid,a nd M. Wormserf or valuablet echnicaal ssistance.

Keywords

Elongation factor gene
Genetic polymorphism
Mycoplasma

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10.1111/j.1574-6968.1988.tb02928.x

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title = "Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains",

abstract = "Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.",

keywords = "Elongation factor gene, Genetic polymorphism, Mycoplasma",

author = "D. Yogev and S. Sela and H. Bercovier and S. Razin",

note = "Funding Information: This study was supportedb y a grant from the United States-IsraeBl inationalAgricultural Re-searcha nd DevelopmenFtu nd (BARD). We thank S. Levisohnf or the M. gallisepticumst rains,G .E. Kenny for the M. pneumoniaset rains,N . Brot for providingt he tufA plasmid,a nd M. Wormserf or valuablet echnicaal ssistance.",

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doi = "10.1111/j.1574-6968.1988.tb02928.x",

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AU - Yogev, D.

AU - Sela, S.

AU - Bercovier, H.

AU - Razin, S.

N1 - Funding Information: This study was supportedb y a grant from the United States-IsraeBl inationalAgricultural Re-searcha nd DevelopmenFtu nd (BARD). We thank S. Levisohnf or the M. gallisepticumst rains,G .E. Kenny for the M. pneumoniaset rains,N . Brot for providingt he tufA plasmid,a nd M. Wormserf or valuablet echnicaal ssistance.

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N2 - Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.

AB - Polymorphism in mycoplasma strains was observed by Southern blot hybridization of the digested mycoplasma DNAs with the elongation factor (EF-Tu) gene tuf of Escherichia coli. The hybridization patterns revealed genotypic heterogeneity among Mycoplasma gallisepticum strains and a remarkable degree of homogeneity among Mucoplasma pneumoniae strains isolated from pneumonia patients. The distinction among M. gallisepticum strain clusters achieved by the tuf gene probe corresponded exactly with that obtained with the rRNA gene probe pMC5. The tuf gene probe may thus be added as another effective tool in the taxonomy of Mollicutes and in epidemiological surveys of mycoplasma infections.

KW - Elongation factor gene

KW - Genetic polymorphism

KW - Mycoplasma

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Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains

Abstract

Bibliographical note

Keywords

UN SDGs

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