Endo-β-glucosidase from Aspergillus niger grown on a monoterpene glycoside-containing medium

Oded Shoseyov, Ben Ami Bravdo, Raphael Ikan, Ilan Chet

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39 Scopus citations


An endo-β-glucosidase was isolated from Aspergillus niger grown on a medium containing rutin as the sole carbon source, and was partially purified by affinity chromatography. The enzyme was found to be extracellular. Its optimum pH was 3.4, and its optimum temperature 65°. KM values were 1 mM (PNPG) and 1.25 mM (geranyl-β-rutinoside), Vmax values were 0.22 μmol/min/mg protein (PNPG) and 0.08 μmol/min/mg protein (geranyl-β-rutinoside), and KI was 40 mM (glucose). The enzyme's activity was not inhibited by fructose, sucrose or SO2, and was enhanced by ethanol. No loss of activity was found after seven days at 50°. Mr determined by gel filtration was 120 000. Using polyacrylamide isoelectric focusing gel, a pI of 3.9 was obtained.

Original languageAmerican English
Pages (from-to)1973-1976
Number of pages4
Issue number7
StatePublished - 1988


  • Aspergillus niger, Vitis vinifera
  • monoterpene glycosides
  • wine
  • β-glucosidase.


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