Endogenous "ouabain-like" activity in bovine plasma

Daniel M. Tal, Silvia Katchalsky, David Lichtstein, Steven J.D. Karlish*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

An endogenous inhibitor of the sodium pump has been detected and concentrated 1000-fold from bovine plasma. The steps of purification included deproteinization and extraction with methanol, removal of lipids by coextractions with a lipopnilic solvent, desalting and further concentration by adsorption on C18-SepPack cartridges and HPLC fractionation on a weak anionic exchange column. The material isolated displaces 3H-ouabain from brain synaptosomes, inhibits red cell membrane Na,K-ATPase without inhibiting Mg-ATPase or Ca,Mg-ATPase. Deproteinization of plasma by boiling may lead to appearance of non-specific inhibitors. The procedures developed should now permit isolation of sufficient amount of material for further purification and structural characterization.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number1
DOIs
StatePublished - 26 Feb 1986

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