TY - JOUR
T1 - Endogenous "ouabain-like" activity in bovine plasma
AU - Tal, Daniel M.
AU - Katchalsky, Silvia
AU - Lichtstein, David
AU - Karlish, Steven J.D.
PY - 1986/2/26
Y1 - 1986/2/26
N2 - An endogenous inhibitor of the sodium pump has been detected and concentrated 1000-fold from bovine plasma. The steps of purification included deproteinization and extraction with methanol, removal of lipids by coextractions with a lipopnilic solvent, desalting and further concentration by adsorption on C18-SepPack cartridges and HPLC fractionation on a weak anionic exchange column. The material isolated displaces 3H-ouabain from brain synaptosomes, inhibits red cell membrane Na,K-ATPase without inhibiting Mg-ATPase or Ca,Mg-ATPase. Deproteinization of plasma by boiling may lead to appearance of non-specific inhibitors. The procedures developed should now permit isolation of sufficient amount of material for further purification and structural characterization.
AB - An endogenous inhibitor of the sodium pump has been detected and concentrated 1000-fold from bovine plasma. The steps of purification included deproteinization and extraction with methanol, removal of lipids by coextractions with a lipopnilic solvent, desalting and further concentration by adsorption on C18-SepPack cartridges and HPLC fractionation on a weak anionic exchange column. The material isolated displaces 3H-ouabain from brain synaptosomes, inhibits red cell membrane Na,K-ATPase without inhibiting Mg-ATPase or Ca,Mg-ATPase. Deproteinization of plasma by boiling may lead to appearance of non-specific inhibitors. The procedures developed should now permit isolation of sufficient amount of material for further purification and structural characterization.
UR - http://www.scopus.com/inward/record.url?scp=0022507270&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(86)90934-4
DO - 10.1016/0006-291X(86)90934-4
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C2 - 3006675
AN - SCOPUS:0022507270
SN - 0006-291X
VL - 135
SP - 1
EP - 8
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -