Endoreduplication and polyploidy in fragile X cells induced by methotrexate and fluorodeoxyuridine: Implications for diagnosis

C. Kimchi-Sarfaty; R. Goitein; B. Kerem; M. Werner; B. Meidan; T. Schaap

doi:10.1002/ajmg.1320380257

Endoreduplication and polyploidy in fragile X cells induced by methotrexate and fluorodeoxyuridine: Implications for diagnosis

C. Kimchi-Sarfaty, R. Goitein, B. Kerem, M. Werner, B. Meidan, T. Schaap^*

^*Corresponding author for this work

Department of Genetics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Lymphoblastoid cell lines from fragile X patients and amniotic cells from fragile X embryos, when cultured with methotrexate (MTX) or fluorodeoxyuridine (FUdR), showed a significant increase in endoreduplication and polyploidy. This phenomenon was not observed in fragile X lymphocytes or in lymphoblastoid cell lines and amniotic cells of normal control individuals. The relationship between the inducible fragile site at Xq27.3 and the inducible endoreduplication is discussed. The induction of endoreduplication and polyploidy in fragile X lymphoblasts and amniocytes is evaluated as a possible diagnostic test.

Original language	English
Pages (from-to)	429-433
Number of pages	5
Journal	American Journal of Medical Genetics
Volume	38
Issue number	2-3
DOIs	https://doi.org/10.1002/ajmg.1320380257
State	Published - 1991

Keywords

amniocytes
endoreduplication polyploidy
fragile Xq27.3
lymphoblasts

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abstract = "Lymphoblastoid cell lines from fragile X patients and amniotic cells from fragile X embryos, when cultured with methotrexate (MTX) or fluorodeoxyuridine (FUdR), showed a significant increase in endoreduplication and polyploidy. This phenomenon was not observed in fragile X lymphocytes or in lymphoblastoid cell lines and amniotic cells of normal control individuals. The relationship between the inducible fragile site at Xq27.3 and the inducible endoreduplication is discussed. The induction of endoreduplication and polyploidy in fragile X lymphoblasts and amniocytes is evaluated as a possible diagnostic test.",

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AU - Kimchi-Sarfaty, C.

AU - Goitein, R.

AU - Kerem, B.

AU - Werner, M.

AU - Meidan, B.

AU - Schaap, T.

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AB - Lymphoblastoid cell lines from fragile X patients and amniotic cells from fragile X embryos, when cultured with methotrexate (MTX) or fluorodeoxyuridine (FUdR), showed a significant increase in endoreduplication and polyploidy. This phenomenon was not observed in fragile X lymphocytes or in lymphoblastoid cell lines and amniotic cells of normal control individuals. The relationship between the inducible fragile site at Xq27.3 and the inducible endoreduplication is discussed. The induction of endoreduplication and polyploidy in fragile X lymphoblasts and amniocytes is evaluated as a possible diagnostic test.

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Endoreduplication and polyploidy in fragile X cells induced by methotrexate and fluorodeoxyuridine: Implications for diagnosis

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