TY - JOUR
T1 - Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation
AU - Nieuwenhuis, F. J.R.M.
AU - Kanner, B. I.
AU - Gutnick, D. L.
AU - Postma, P. W.
AU - Van Dam, K.
PY - 1973/10/19
Y1 - 1973/10/19
N2 - 1. 1. Energy conservation in membranes of Escherichia coli was measured using 9-amino-6-chloro-2-methoxyacridine fluorescence. 2. 2. Energy conservation in membranes, depleted of coupling factor, can be restored either by the isolated crude coupling factor or by N,N′-dicyclohexylcarbodiimide (DCCD) but not by Dio-9 or oligomycin. 3. 3. Several mutants, defective in different components of the ATP-synthesizing complex, are described. 4. 4. Mutants possessing a DCCD-insensitive ATPase have been isolated which can or cannot be recoupled by DCCD.
AB - 1. 1. Energy conservation in membranes of Escherichia coli was measured using 9-amino-6-chloro-2-methoxyacridine fluorescence. 2. 2. Energy conservation in membranes, depleted of coupling factor, can be restored either by the isolated crude coupling factor or by N,N′-dicyclohexylcarbodiimide (DCCD) but not by Dio-9 or oligomycin. 3. 3. Several mutants, defective in different components of the ATP-synthesizing complex, are described. 4. 4. Mutants possessing a DCCD-insensitive ATPase have been isolated which can or cannot be recoupled by DCCD.
UR - http://www.scopus.com/inward/record.url?scp=0015910049&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(73)90151-5
DO - 10.1016/0005-2728(73)90151-5
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C2 - 4149157
AN - SCOPUS:0015910049
SN - 0005-2728
VL - 325
SP - 62
EP - 71
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 1
ER -