Engineering a bifunctional starch-cellulose cross-bridge protein

Ilan Levy, Tzur Paldi, Oded Shoseyov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Biodegradable starch- and cellulose-based polymers have a range of properties which make them suitable for use in a wide array of biomedical applications ranging from bone replacement to engineering of tissue scaffolds and drug delivery systems. A novel polysaccharide cross-bridging protein was designed which was comprised of a cellulose-binding domain from Clostridium cellulovorans (CBDclos) and a starch-binding domain from Aspergillus niger B1 (SBDAsp). The two genes were fused in-frame via a synthetic elastin gene to construct a Cellulose/Starch Cross bridging Protein (CSCP). Recombinant CSCP was expressed in Escherichia coli, and successfully refolded from inclusion bodies. CSCP demonstrated cross-bridging ability in different model systems composed of insoluble or soluble starch and cellulose. The aspect that different carbohydrate-binding module maintain their binding capacity over a wide range of conditions, without the need for chemical reactions, makes them attractive domains for designing new classes of chimeric polysaccharide-binding domains which demonstrate potential for use in a wide range of biomaterials.

Original languageEnglish
Pages (from-to)1841-1849
Number of pages9
JournalBiomaterials
Volume25
Issue number10
DOIs
StatePublished - May 2004

Keywords

  • Cellulose-binding domain
  • Cross-bridging
  • Starch-binding domain

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