Abstract
Biodegradable starch- and cellulose-based polymers have a range of properties which make them suitable for use in a wide array of biomedical applications ranging from bone replacement to engineering of tissue scaffolds and drug delivery systems. A novel polysaccharide cross-bridging protein was designed which was comprised of a cellulose-binding domain from Clostridium cellulovorans (CBDclos) and a starch-binding domain from Aspergillus niger B1 (SBDAsp). The two genes were fused in-frame via a synthetic elastin gene to construct a Cellulose/Starch Cross bridging Protein (CSCP). Recombinant CSCP was expressed in Escherichia coli, and successfully refolded from inclusion bodies. CSCP demonstrated cross-bridging ability in different model systems composed of insoluble or soluble starch and cellulose. The aspect that different carbohydrate-binding module maintain their binding capacity over a wide range of conditions, without the need for chemical reactions, makes them attractive domains for designing new classes of chimeric polysaccharide-binding domains which demonstrate potential for use in a wide range of biomaterials.
Original language | English |
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Pages (from-to) | 1841-1849 |
Number of pages | 9 |
Journal | Biomaterials |
Volume | 25 |
Issue number | 10 |
DOIs | |
State | Published - May 2004 |
Keywords
- Cellulose-binding domain
- Cross-bridging
- Starch-binding domain