Enhanced osteopontin expression in a murine model of allergen-induced airway remodelling

M. Kohan, R. Bader, I. Puxeddu, F. Levi-Schaffer, R. Breuer, N. Berkman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Background: Airway remodelling is a central pathophysiological feature of chronic asthma. A wide variety of cytokines and growth factors are likely to be involved in the development of airway remodelling. Osteopontin (OPN) is a cytokine with pro-fibrotic properties; however, its role in airway remodelling in asthma has not been explored. Objective: To determine the expression and cellular sources of OPN in a murine model of chronic allergen-induced airway remodelling. Methods: BALB/c mice were sensitized and exposed to ovalbumin (OVA) or saline inhalations for 5 weeks and killed 24 h after the last inhalation. The following parameters of inflammation and remodelling were assessed: differential cell counts in bronchoalveolar lavage (BAL) fluid lung collagen content (colorimetric biochemical assay) and peribronchial smooth muscle content (immunohistochemistry, followed by image analysis). OPN expression in BAL and lung tissue was determined by PCR and ELISA. The cellular source and distribution of OPN were evaluated by immunohistochemistry and immunofluorescence. Results: OPN expression is up-regulated in lung tissue and in BAL fluid of OVA-treated mice and correlates with collagen content and peribronchial smooth muscle area. In addition, OPN significantly increases collagen deposition in vitro in a murine lung cell line. Cells producing OPN include the airway epithelium and cells of the submucosal inflammatory infiltrate (T cells, eosinophils, and macrophages). Positive staining for OPN was also observed in bronchial tissue from human asthmatic subjects. Conclusion: OPN expression in the lungs is increased in a murine model of allergen-induced chronic airway remodelling, suggesting a role for this cytokine in airway remodelling in asthma.

Original languageAmerican English
Pages (from-to)1444-1454
Number of pages11
JournalClinical and Experimental Allergy
Volume37
Issue number10
DOIs
StatePublished - Oct 2007

Keywords

  • Allergen-induced airway remodelling
  • Asthma
  • Osteopontin

Fingerprint

Dive into the research topics of 'Enhanced osteopontin expression in a murine model of allergen-induced airway remodelling'. Together they form a unique fingerprint.

Cite this