Enhancement of laccase activity in liquid cultures of the ligninolytic fungus Pleurotus ostreatus by cotton stalk extract

Orly Ardon, Zohar Kerem, Yitzhak Hadar*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

73 Scopus citations

Abstract

Laccase is one of the enzymes suggested to play a role in fungal ligninolytic systems. Cotton stalks were found to be an excellent substrate for Pleurotus cultivation. In this work, the effects of cotton-stalk extract (CSE) were studied. It was found to stimulate both fungal growth in surface cultures and extracellular laccase activity in submerged cultures. Induction of laccase activity was dependent upon the concentration of the supplemented extract. The stimulation of laccase was observed as early as 3 h after amendment of CSE. Laccase activity staining after separation by gel electrophoresis revealed two major bands in both control and the CSE-induced cultures.

Original languageAmerican English
Pages (from-to)201-207
Number of pages7
JournalJournal of Biotechnology
Volume51
Issue number3
DOIs
StatePublished - 15 Nov 1996
EventProceedings of the 1995 Workshop on Environmental Biotechnology -
Duration: 28 Nov 199528 Nov 1995

Bibliographical note

Funding Information:
We thank Dana Friesem and Barbara Gorodecki for their help and stimulating discussions. This work was supported by the Agency for International Development (AID-CDR) and the Charles Wolfson Charitable Trust.

Keywords

  • Pleurotus
  • laccase
  • lignin degradation
  • mushroom
  • white rot fungi

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