TY - JOUR
T1 - Enhancement of the invasive ability of a transformed human bronchial epithelial cell line by 12-o-tetra-decanoyl-phorbol-13-acetate and diacylglycerol
AU - Bonfil, R. Daniel
AU - Momiki, Shigeru
AU - Fridman, Rafael
AU - Reich, Reuven
AU - Reddel, Roger
AU - Harris, Curtis C.
AU - Klien-szanto, Andres
PY - 1989/12
Y1 - 1989/12
N2 - The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Intraluminal TPA treatment caused increased BEAS-2B cell proliferation and downgrowth into the tracheal stroma. In an in vitro invasion assay, TPA enhanced the invasive capacity of BEAS-2B cells 20- to 25-fold. A similar result was observed with diacylglycerol (DAG), an endogenous activator of protein kinase C, and the effects of TPA and DAG were abolished by simultaneous treatment with H-7, a protein kinase C inhibitor. TPA induced type IV collagenolysis, and this effect also was prevented by H-7. These data are consistent with the hypothesis that TPA causes these cells to become invasive by inducing collagenase activity and that this effect is mediated via protein kinase C.
AB - The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Intraluminal TPA treatment caused increased BEAS-2B cell proliferation and downgrowth into the tracheal stroma. In an in vitro invasion assay, TPA enhanced the invasive capacity of BEAS-2B cells 20- to 25-fold. A similar result was observed with diacylglycerol (DAG), an endogenous activator of protein kinase C, and the effects of TPA and DAG were abolished by simultaneous treatment with H-7, a protein kinase C inhibitor. TPA induced type IV collagenolysis, and this effect also was prevented by H-7. These data are consistent with the hypothesis that TPA causes these cells to become invasive by inducing collagenase activity and that this effect is mediated via protein kinase C.
UR - http://www.scopus.com/inward/record.url?scp=0024367971&partnerID=8YFLogxK
U2 - 10.1093/carcin/10.12.2335
DO - 10.1093/carcin/10.12.2335
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C2 - 2556220
AN - SCOPUS:0024367971
SN - 0143-3334
VL - 10
SP - 2335
EP - 2338
JO - Carcinogenesis
JF - Carcinogenesis
IS - 12
ER -