TY - JOUR
T1 - Enzyme-capped relay-functionalized mesoporous carbon nanoparticles
T2 - Effective bioelectrocatalytic matrices for sensing and biofuel cell applications
AU - Trifonov, Alexander
AU - Herkendell, Katharina
AU - Tel-Vered, Ran
AU - Yehezkeli, Omer
AU - Woerner, Michael
AU - Willner, Itamar
PY - 2013/12/23
Y1 - 2013/12/23
N2 - The porous high surface area and conducting properties of mesoporous carbon nanoparticles, CNPs (<500 nm diameter of NPs, pore dimensions ∼6.3 nm), are implemented to design electrically contacted enzyme electrodes for biosensing and biofuel cell applications. The relay units ferrocene methanol, Fc-MeOH, methylene blue, MB+, and 2,2′-azinobis(3- ethylbenzothiazoline-6-sulfonic acid), ABTS2-, are loaded in the pores of the mesoporous CNPs, and the pores are capped with glucose oxidase, GOx, horseradish peroxidase, HRP, or bilirubin oxidase, BOD, respectively. The resulting relay/enzyme-functionalized CNPs are immobilized on glassy carbon electrodes, and the relays encapsulated in the pores are sufficiently free to electrically contact the different enzymes with the bulk electrode supports. The Fc-MeOH/GOx CNP-functionalized electrode is implemented for the bioelectrocatalyzed sensing of glucose, and the MB+/HRP-modified CNPs are applied for the electrochemical sensing of H2O2. The ABTS2-/BOD-modified CNPs provide an effective electrically contacted material for the bioelectrocatalyzed reduction of O2 (kcat = 94 electrons·s-1). Integration of the Fc-MeOH/GOx CNP electrode and of the electrically wired ABTS2-/BOD CNP electrode as anode and cathode, respectively, yields a biofuel cell revealing a power output of ∼95 μW·cm-2.
AB - The porous high surface area and conducting properties of mesoporous carbon nanoparticles, CNPs (<500 nm diameter of NPs, pore dimensions ∼6.3 nm), are implemented to design electrically contacted enzyme electrodes for biosensing and biofuel cell applications. The relay units ferrocene methanol, Fc-MeOH, methylene blue, MB+, and 2,2′-azinobis(3- ethylbenzothiazoline-6-sulfonic acid), ABTS2-, are loaded in the pores of the mesoporous CNPs, and the pores are capped with glucose oxidase, GOx, horseradish peroxidase, HRP, or bilirubin oxidase, BOD, respectively. The resulting relay/enzyme-functionalized CNPs are immobilized on glassy carbon electrodes, and the relays encapsulated in the pores are sufficiently free to electrically contact the different enzymes with the bulk electrode supports. The Fc-MeOH/GOx CNP-functionalized electrode is implemented for the bioelectrocatalyzed sensing of glucose, and the MB+/HRP-modified CNPs are applied for the electrochemical sensing of H2O2. The ABTS2-/BOD-modified CNPs provide an effective electrically contacted material for the bioelectrocatalyzed reduction of O2 (kcat = 94 electrons·s-1). Integration of the Fc-MeOH/GOx CNP electrode and of the electrically wired ABTS2-/BOD CNP electrode as anode and cathode, respectively, yields a biofuel cell revealing a power output of ∼95 μW·cm-2.
KW - bilirubin oxidase
KW - electrical wiring
KW - glucose
KW - mesoporous carbon
KW - oxygen reduction
KW - sensor
UR - http://www.scopus.com/inward/record.url?scp=84891353429&partnerID=8YFLogxK
U2 - 10.1021/nn405218x
DO - 10.1021/nn405218x
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C2 - 24266869
AN - SCOPUS:84891353429
SN - 1936-0851
VL - 7
SP - 11358
EP - 11368
JO - ACS Nano
JF - ACS Nano
IS - 12
ER -