Enzyme-capped relay-functionalized mesoporous carbon nanoparticles: Effective bioelectrocatalytic matrices for sensing and biofuel cell applications

Alexander Trifonov, Katharina Herkendell, Ran Tel-Vered, Omer Yehezkeli, Michael Woerner, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

The porous high surface area and conducting properties of mesoporous carbon nanoparticles, CNPs (<500 nm diameter of NPs, pore dimensions ∼6.3 nm), are implemented to design electrically contacted enzyme electrodes for biosensing and biofuel cell applications. The relay units ferrocene methanol, Fc-MeOH, methylene blue, MB+, and 2,2′-azinobis(3- ethylbenzothiazoline-6-sulfonic acid), ABTS2-, are loaded in the pores of the mesoporous CNPs, and the pores are capped with glucose oxidase, GOx, horseradish peroxidase, HRP, or bilirubin oxidase, BOD, respectively. The resulting relay/enzyme-functionalized CNPs are immobilized on glassy carbon electrodes, and the relays encapsulated in the pores are sufficiently free to electrically contact the different enzymes with the bulk electrode supports. The Fc-MeOH/GOx CNP-functionalized electrode is implemented for the bioelectrocatalyzed sensing of glucose, and the MB+/HRP-modified CNPs are applied for the electrochemical sensing of H2O2. The ABTS2-/BOD-modified CNPs provide an effective electrically contacted material for the bioelectrocatalyzed reduction of O2 (kcat = 94 electrons·s-1). Integration of the Fc-MeOH/GOx CNP electrode and of the electrically wired ABTS2-/BOD CNP electrode as anode and cathode, respectively, yields a biofuel cell revealing a power output of ∼95 μW·cm-2.

Original languageEnglish
Pages (from-to)11358-11368
Number of pages11
JournalACS Nano
Volume7
Issue number12
DOIs
StatePublished - 23 Dec 2013

Keywords

  • bilirubin oxidase
  • electrical wiring
  • glucose
  • mesoporous carbon
  • oxygen reduction
  • sensor

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