TY - JOUR
T1 - Enzyme levels used to monitor pesticide resistance in Varroa jacobsoni
AU - Gerson, Uri
AU - Mozes-Koch, Rita
AU - Cohen, Ephraim
PY - 1991
Y1 - 1991
N2 - The contingency of pesticide resistance in the mite Varroa jacobsoni Oudemans prompted this preliminary study, in which methodology for assaying levels of two detoxification systems, namely esterases and glutathione S-transferase (GST), was developed. The total esterolytic activity detected in post- mitochondrial fractions of a homogenized 200-mg sample of V. jacobsoni from 10 colonies was 0.0068 (OD) min −1 μ.g−1 protein. GST activity, expressed in μmol conjugate min−1 μg−1 protein was 0.260±0.21 in the post-mitochondrial supernatant and 0.646±0.48 in the cytosol. These results pertain only to mites obtained from a single experimental apiary of A. mellifera ligustica in Israel. The methodology and preliminary results obtained may be used for subsequent local or regional monitoring of enzyme levels associated with pesticide resistance in V. jacobsoni.
AB - The contingency of pesticide resistance in the mite Varroa jacobsoni Oudemans prompted this preliminary study, in which methodology for assaying levels of two detoxification systems, namely esterases and glutathione S-transferase (GST), was developed. The total esterolytic activity detected in post- mitochondrial fractions of a homogenized 200-mg sample of V. jacobsoni from 10 colonies was 0.0068 (OD) min −1 μ.g−1 protein. GST activity, expressed in μmol conjugate min−1 μg−1 protein was 0.260±0.21 in the post-mitochondrial supernatant and 0.646±0.48 in the cytosol. These results pertain only to mites obtained from a single experimental apiary of A. mellifera ligustica in Israel. The methodology and preliminary results obtained may be used for subsequent local or regional monitoring of enzyme levels associated with pesticide resistance in V. jacobsoni.
KW - Apis mellifera
KW - Detoxification systems
KW - Esterases
KW - Glutathione S-transferase
KW - Honey bees
KW - Pesticide resistance
KW - Varroa jacobsoni
UR - http://www.scopus.com/inward/record.url?scp=0008606664&partnerID=8YFLogxK
U2 - 10.1080/00218839.1991.11101229
DO - 10.1080/00218839.1991.11101229
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AN - SCOPUS:0008606664
SN - 0021-8839
VL - 30
SP - 17
EP - 20
JO - Journal of Apicultural Research
JF - Journal of Apicultural Research
IS - 1
ER -