Escherichia coli produces linoleic acid during late stationary phase

H. D. Rabinowitch, D. Sklan, D. H. Chace, R. D. Stevens, I. Fridovich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin- layer chromatography and was measured as the methyl ester by gas chromatography. The linoleic acid methyl ester was identified by its mass spectrum. Lipids extracted from late-stationary-phase cells generated thiobarbituric acid-reactive carbonyl products when incubated with a free radical initiator. In contrast, extracts from log-phase or early-stationary- phase cells failed to do so, in accordance with the presence of polyunsaturated fatty acid only in the stationary-phase cells.

Original languageEnglish
Pages (from-to)5324-5328
Number of pages5
JournalJournal of Bacteriology
Volume175
Issue number17
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Dive into the research topics of 'Escherichia coli produces linoleic acid during late stationary phase'. Together they form a unique fingerprint.

Cite this