Abstract
Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin- layer chromatography and was measured as the methyl ester by gas chromatography. The linoleic acid methyl ester was identified by its mass spectrum. Lipids extracted from late-stationary-phase cells generated thiobarbituric acid-reactive carbonyl products when incubated with a free radical initiator. In contrast, extracts from log-phase or early-stationary- phase cells failed to do so, in accordance with the presence of polyunsaturated fatty acid only in the stationary-phase cells.
Original language | English |
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Pages (from-to) | 5324-5328 |
Number of pages | 5 |
Journal | Journal of Bacteriology |
Volume | 175 |
Issue number | 17 |
DOIs | |
State | Published - 1993 |
Externally published | Yes |