TY - JOUR
T1 - Escherichia coli response to hydrogen peroxide
T2 - A role for DNA supercoiling, Topoisomerase I and Fis
AU - Weinstein-Fischer, Dalit
AU - Elgrably-Weiss, Maya
AU - Altuvia, Shoshy
PY - 2000
Y1 - 2000
N2 - Bacterial cells respond to the deleterious effects of reactive oxygen species by inducing the expression of antioxidant defence genes. Here we show that treatment with hydrogen peroxide leads to a transient decrease in DNA negative supercoiling. We also report that hydrogen peroxide activates topA P1 promoter expression. The peroxide-dependent topA P1 activation is independent of oxyR, but is mediated by Fis. This nucleoid-associated protein binds to the promoter region of topA. We also show that a fis deficient mutant strain is extremely sensitive to hydrogen peroxide. Our results suggest that topA activation by Fis is an important component of the Escherichia coli response to oxidative stress.
AB - Bacterial cells respond to the deleterious effects of reactive oxygen species by inducing the expression of antioxidant defence genes. Here we show that treatment with hydrogen peroxide leads to a transient decrease in DNA negative supercoiling. We also report that hydrogen peroxide activates topA P1 promoter expression. The peroxide-dependent topA P1 activation is independent of oxyR, but is mediated by Fis. This nucleoid-associated protein binds to the promoter region of topA. We also show that a fis deficient mutant strain is extremely sensitive to hydrogen peroxide. Our results suggest that topA activation by Fis is an important component of the Escherichia coli response to oxidative stress.
UR - http://www.scopus.com/inward/record.url?scp=0034065133&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2958.2000.01805.x
DO - 10.1046/j.1365-2958.2000.01805.x
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C2 - 10760142
AN - SCOPUS:0034065133
SN - 0950-382X
VL - 35
SP - 1413
EP - 1420
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 6
ER -