TY - JOUR
T1 - Essential oils from Artemisia judaica, Ruta graveolens and Salvia palaestina with antiradical, cytotoxic and AMPA receptor-modulatory activities
AU - Qneibi, Mohammad
AU - Jaradat, Nidal
AU - Hamed, Othman
AU - Hawash, Mohammed
AU - Abualhasan, Murad
AU - Qadi, Mohammad
AU - Bdir, Sosana
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/12
Y1 - 2025/12
N2 - Essential oils (EOs) from the leaves of Artemisia judaica, Ruta graveolens, and Salvia palaestina were analyzed for their phytochemical composition and their antiradical, cytotoxic, and neuromodulatory activities. Using microwave-ultrasonic technology, EOs were extracted from S. palaestina, A. judaica, and R. graveolens, and their antiradical activity (via DPPH assay), cytotoxicity, and neuromodulatory effects (using whole-cell patch-clamp recordings) were evaluated. The main compounds that were explored by GC-MS analysis include piperitone and carvacrol in A. Judaica, 2-undecanone in R. graveolens, and carvacrol in S. palaestina. S. palaestina exhibited the greatest antiradical effect with a 50% inhibition concentration of 8.30 µg/mL. Cytotoxicity tests showed that these EOs express dose-dependent activity on HeLa, HepG3, and B16F1 cancer cells. Among them, S. palaestina and A. judaica were the most potent ones. Patch-clamp recordings showed that S. palaestina was the most potent in inhibiting action toward AMPA receptors, thereby reducing currents up to 6-fold and modulating receptor kinetics. A. Judaica and R. graveolens also inhibited the AMPA receptor activity, but to a lesser extent. These findings point to the important bioactive potential of these EOs, especially S. palaestina, which demonstrated high antiradical and AMPA receptor-modulatory activities and, thus, could be of interest for pharmaceutical and neuroprotective uses.
AB - Essential oils (EOs) from the leaves of Artemisia judaica, Ruta graveolens, and Salvia palaestina were analyzed for their phytochemical composition and their antiradical, cytotoxic, and neuromodulatory activities. Using microwave-ultrasonic technology, EOs were extracted from S. palaestina, A. judaica, and R. graveolens, and their antiradical activity (via DPPH assay), cytotoxicity, and neuromodulatory effects (using whole-cell patch-clamp recordings) were evaluated. The main compounds that were explored by GC-MS analysis include piperitone and carvacrol in A. Judaica, 2-undecanone in R. graveolens, and carvacrol in S. palaestina. S. palaestina exhibited the greatest antiradical effect with a 50% inhibition concentration of 8.30 µg/mL. Cytotoxicity tests showed that these EOs express dose-dependent activity on HeLa, HepG3, and B16F1 cancer cells. Among them, S. palaestina and A. judaica were the most potent ones. Patch-clamp recordings showed that S. palaestina was the most potent in inhibiting action toward AMPA receptors, thereby reducing currents up to 6-fold and modulating receptor kinetics. A. Judaica and R. graveolens also inhibited the AMPA receptor activity, but to a lesser extent. These findings point to the important bioactive potential of these EOs, especially S. palaestina, which demonstrated high antiradical and AMPA receptor-modulatory activities and, thus, could be of interest for pharmaceutical and neuroprotective uses.
KW - AMPA receptor
KW - Antiradical
KW - Cytotoxicity
KW - Desensitization
KW - Phytochemical composition
UR - https://www.scopus.com/pages/publications/105026272263
U2 - 10.1038/s41598-025-30649-3
DO - 10.1038/s41598-025-30649-3
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C2 - 41315747
AN - SCOPUS:105026272263
SN - 2045-2322
VL - 15
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 45644
ER -