Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

M. Steinitz; I. Seppala; K. Eichmann; G. Klein

doi:10.1016/s0340-904x(79)80060-3

Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

M. Steinitz
, I. Seppala
, K. Eichmann
, G. Klein

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T₄-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

Original language	English
Pages (from-to)	41-47
Number of pages	7
Journal	Immunobiology
Volume	156
Issue number	1-2
DOIs	https://doi.org/10.1016/s0340-904x(79)80060-3
State	Published - 1979
Externally published	Yes

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10.1016/s0340-904x(79)80060-3

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title = "Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate",

abstract = "A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.",

author = "M. Steinitz and I. Seppala and K. Eichmann and G. Klein",

year = "1979",

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AU - Eichmann, K.

AU - Klein, G.

PY - 1979

Y1 - 1979

N2 - A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

AB - A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

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ER -

Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

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