Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy

Anders Österborg; Michael Steinitz; Nongnit Lewin; Susanne Bergenbrant; Göran Holm; Ann Kari Lefvert; Håkan Mellstedt

doi:10.1182/blood.v78.10.2642.2642

Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy

Anders Österborg
, Michael Steinitz
, Nongnit Lewin
, Susanne Bergenbrant
, Göran Holm
, Ann Kari Lefvert
, Håkan Mellstedt^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

B-lymphocyte clones bearing an idiotypic lg structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id⁺/CD19⁺/CD20⁺/CD21⁺ or id⁺/CD19⁺/CD20⁺/CD21^- . Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocvte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same lg heavy chain gene rearrangements in Southern blot analysis using a J_H probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.

Original language	English
Pages (from-to)	2642-2649
Number of pages	8
Journal	Blood
Volume	78
Issue number	10
DOIs	https://doi.org/10.1182/blood.v78.10.2642.2642
State	Published - 15 Nov 1991

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10.1182/blood.v78.10.2642.2642

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title = "Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy",

abstract = "B-lymphocyte clones bearing an idiotypic lg structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21- . Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocvte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same lg heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.",

author = "Anders {\"O}sterborg and Michael Steinitz and Nongnit Lewin and Susanne Bergenbrant and G{\"o}ran Holm and Lefvert, \{Ann Kari\} and H{\aa}kan Mellstedt",

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doi = "10.1182/blood.v78.10.2642.2642",

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AU - Österborg, Anders

AU - Steinitz, Michael

AU - Lewin, Nongnit

AU - Bergenbrant, Susanne

AU - Holm, Göran

AU - Lefvert, Ann Kari

AU - Mellstedt, Håkan

PY - 1991/11/15

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N2 - B-lymphocyte clones bearing an idiotypic lg structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21- . Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocvte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same lg heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.

AB - B-lymphocyte clones bearing an idiotypic lg structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21- . Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocvte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same lg heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.

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Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy

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