Establishment of methylation patterns in ES cells

Ofra Sabag, Ayelet Zamir, Ilana Keshet, Merav Hecht, Guy Ludwig, Amalia Tabib, Joshua Moss, Howard Cedar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


After erasure in the early animal embryo, a new bimodal DNA methylation pattern is regenerated at implantation. We have identified a demethylation pathway in mouse embryonic cells that uses hydroxymethylation (Tet1), deamination (Aid), glycosylation (Mbd4) and excision repair (Gadd45a) genes. Surprisingly, this demethylation system is not necessary for generating the overall bimodal methylation pattern but does appear to be involved in resetting methylation patterns during somatic-cell reprogramming.

Original languageAmerican English
Pages (from-to)110-112
Number of pages3
JournalNature Structural and Molecular Biology
Issue number1
StatePublished - Jan 2014

Bibliographical note

Funding Information:
Knockout Aid− and Mbd4− ES cells were obtained from T. Honjo (Kyoto University) and A. Bird (Edinburgh University), respectively. Tissue DNA from Aid− and Mbd4− mice was obtained from W. Reik (Cambridge University) and A. Bird (Edinburgh University), respectively. This research was supported by US National Institutes of Health grant 5R01GM062275 (H.C.), European Research Council grant ERCGA268614 (H.C.), Israeli Centers Of Research Excellence grant 41/11 (H.C.), Israel Cancer Research Foundation grant 210910 (H.C.) and Israel Science Foundation grant 419/10 (H.C.).


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