Ethacrynic acid influx and efflux in kidney cortex slices: Dependence on sodium gradient

Yeda Gutman*, Hanna Wald, Walter Czaczkes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Ethacrynic acid (EA) accumulation in rat kidney cortex slices was inhibited by reduction of sodium concentration in the incubation medium. Preloading of slices with sodium reduced EA accumulation at medium sodium concentration of 30-140 mM. Ouabain (10-3 M) inhibited EA accumulation in kidney cortex slices of rabbit, guinea-pig, Psammomys obesus, rat, mouse and Acomys cahirinus in decreasing order. Ouabain inhibited the sodium pump in kidney cortex slices of these species in the same order. Ethacrynic acid efflux was faster from slices of rat kidney medulla than from kidney cortex. the efflux rate from cortical slices increased when sodium concentration in the incubation medium was lowered. Probenecid (10-3 M) in the medium enhanced the efflux rate of EA from kidney cortex slices. It was concluded that EA fluxes in kidney cortex slices fit the model of a sodiumactivated carrier mechanism and that the sodium gradient across the cell membrane determines the direction of net EA transport.

Original languageEnglish
Pages (from-to)2171-2175
Number of pages5
JournalBiochemical Pharmacology
Volume27
Issue number17
DOIs
StatePublished - 1978

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