TY - JOUR
T1 - Ethacrynic acid influx and efflux in kidney cortex slices
T2 - Dependence on sodium gradient
AU - Gutman, Yeda
AU - Wald, Hanna
AU - Czaczkes, Walter
PY - 1978
Y1 - 1978
N2 - Ethacrynic acid (EA) accumulation in rat kidney cortex slices was inhibited by reduction of sodium concentration in the incubation medium. Preloading of slices with sodium reduced EA accumulation at medium sodium concentration of 30-140 mM. Ouabain (10-3 M) inhibited EA accumulation in kidney cortex slices of rabbit, guinea-pig, Psammomys obesus, rat, mouse and Acomys cahirinus in decreasing order. Ouabain inhibited the sodium pump in kidney cortex slices of these species in the same order. Ethacrynic acid efflux was faster from slices of rat kidney medulla than from kidney cortex. the efflux rate from cortical slices increased when sodium concentration in the incubation medium was lowered. Probenecid (10-3 M) in the medium enhanced the efflux rate of EA from kidney cortex slices. It was concluded that EA fluxes in kidney cortex slices fit the model of a sodiumactivated carrier mechanism and that the sodium gradient across the cell membrane determines the direction of net EA transport.
AB - Ethacrynic acid (EA) accumulation in rat kidney cortex slices was inhibited by reduction of sodium concentration in the incubation medium. Preloading of slices with sodium reduced EA accumulation at medium sodium concentration of 30-140 mM. Ouabain (10-3 M) inhibited EA accumulation in kidney cortex slices of rabbit, guinea-pig, Psammomys obesus, rat, mouse and Acomys cahirinus in decreasing order. Ouabain inhibited the sodium pump in kidney cortex slices of these species in the same order. Ethacrynic acid efflux was faster from slices of rat kidney medulla than from kidney cortex. the efflux rate from cortical slices increased when sodium concentration in the incubation medium was lowered. Probenecid (10-3 M) in the medium enhanced the efflux rate of EA from kidney cortex slices. It was concluded that EA fluxes in kidney cortex slices fit the model of a sodiumactivated carrier mechanism and that the sodium gradient across the cell membrane determines the direction of net EA transport.
UR - http://www.scopus.com/inward/record.url?scp=0018174661&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(78)90291-5
DO - 10.1016/0006-2952(78)90291-5
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C2 - 728168
AN - SCOPUS:0018174661
SN - 0006-2952
VL - 27
SP - 2171
EP - 2175
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 17
ER -