Eukaryotic RNase P: Role of RNA and protein subunits of a primordial catalytic ribonucleoprotein in RNA-based catalysis

Hagit Mann; Yitzhak Ben-Asouli; Aleks Schein; Sana Moussa; Nayef Jarrous

doi:10.1016/S1097-2765(03)00357-5

Eukaryotic RNase P: Role of RNA and protein subunits of a primordial catalytic ribonucleoprotein in RNA-based catalysis

Hagit Mann
, Yitzhak Ben-Asouli
, Aleks Schein
, Sana Moussa
, Nayef Jarrous^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Ribonuclease P (RNase P) is an essential enzyme that processes the 5′ leader sequence of precursor tRNA. Eubacterial RNase P is an RNA enzyme, while its eukaryotic counterpart acts as catalytic ribonucleoprotein, consisting of RNA and numerous protein subunits. To study the latter form, we reconstitute human RNase P activity, demonstrating that the subunits H1 RNA, Rpp21, and Rpp29 are sufficient for 5′ cleavage of precursor tRNA. The reconstituted RNase P precisely delineates its cleavage sites in various substrates and hydrolyzes the phosphodiester bond. Rpp21 and Rpp29 facilitate catalysis by H1 RNA, which seems to require a phylogenetically conserved pseudoknot structure for function. Unexpectedly, Rpp29 forms a catalytic complex with M1 RNA of E. coli RNase P. The results uncover the core components of eukaryotic RNase P, reveal its evolutionary origin in translation, and provide a paradigm for studying RNA-based catalysis by other nuclear and nucleolar ribonucleoprotein enzymes.

Original language	English
Pages (from-to)	925-935
Number of pages	11
Journal	Molecular Cell
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/S1097-2765(03)00357-5
State	Published - Oct 2003

Bibliographical note

Funding Information:
We thank Idit Goldshmidt and Amikam Cohen (Hebrew University) for providing us with yeast nuclear extracts and Cecilia Guerrier-Takada (Yale University) for advising us to set up the 2D-TLC. Y.B.-A. is supported by a postdoctoral fellowship from the Faculty of Medicine, the Hebrew University. This research is supported by the United States-Israel Binational Science Foundation (grant # 2001-017) and Israel Science Foundation (grant #549/01) to N.J. N.J. is the recipient of a Kahanoff Foundation Fellowship.

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title = "Eukaryotic RNase P: Role of RNA and protein subunits of a primordial catalytic ribonucleoprotein in RNA-based catalysis",

abstract = "Ribonuclease P (RNase P) is an essential enzyme that processes the 5′ leader sequence of precursor tRNA. Eubacterial RNase P is an RNA enzyme, while its eukaryotic counterpart acts as catalytic ribonucleoprotein, consisting of RNA and numerous protein subunits. To study the latter form, we reconstitute human RNase P activity, demonstrating that the subunits H1 RNA, Rpp21, and Rpp29 are sufficient for 5′ cleavage of precursor tRNA. The reconstituted RNase P precisely delineates its cleavage sites in various substrates and hydrolyzes the phosphodiester bond. Rpp21 and Rpp29 facilitate catalysis by H1 RNA, which seems to require a phylogenetically conserved pseudoknot structure for function. Unexpectedly, Rpp29 forms a catalytic complex with M1 RNA of E. coli RNase P. The results uncover the core components of eukaryotic RNase P, reveal its evolutionary origin in translation, and provide a paradigm for studying RNA-based catalysis by other nuclear and nucleolar ribonucleoprotein enzymes.",

author = "Hagit Mann and Yitzhak Ben-Asouli and Aleks Schein and Sana Moussa and Nayef Jarrous",

note = "Funding Information: We thank Idit Goldshmidt and Amikam Cohen (Hebrew University) for providing us with yeast nuclear extracts and Cecilia Guerrier-Takada (Yale University) for advising us to set up the 2D-TLC. Y.B.-A. is supported by a postdoctoral fellowship from the Faculty of Medicine, the Hebrew University. This research is supported by the United States-Israel Binational Science Foundation (grant \# 2001-017) and Israel Science Foundation (grant \#549/01) to N.J. N.J. is the recipient of a Kahanoff Foundation Fellowship. ",

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AU - Jarrous, Nayef

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N2 - Ribonuclease P (RNase P) is an essential enzyme that processes the 5′ leader sequence of precursor tRNA. Eubacterial RNase P is an RNA enzyme, while its eukaryotic counterpart acts as catalytic ribonucleoprotein, consisting of RNA and numerous protein subunits. To study the latter form, we reconstitute human RNase P activity, demonstrating that the subunits H1 RNA, Rpp21, and Rpp29 are sufficient for 5′ cleavage of precursor tRNA. The reconstituted RNase P precisely delineates its cleavage sites in various substrates and hydrolyzes the phosphodiester bond. Rpp21 and Rpp29 facilitate catalysis by H1 RNA, which seems to require a phylogenetically conserved pseudoknot structure for function. Unexpectedly, Rpp29 forms a catalytic complex with M1 RNA of E. coli RNase P. The results uncover the core components of eukaryotic RNase P, reveal its evolutionary origin in translation, and provide a paradigm for studying RNA-based catalysis by other nuclear and nucleolar ribonucleoprotein enzymes.

AB - Ribonuclease P (RNase P) is an essential enzyme that processes the 5′ leader sequence of precursor tRNA. Eubacterial RNase P is an RNA enzyme, while its eukaryotic counterpart acts as catalytic ribonucleoprotein, consisting of RNA and numerous protein subunits. To study the latter form, we reconstitute human RNase P activity, demonstrating that the subunits H1 RNA, Rpp21, and Rpp29 are sufficient for 5′ cleavage of precursor tRNA. The reconstituted RNase P precisely delineates its cleavage sites in various substrates and hydrolyzes the phosphodiester bond. Rpp21 and Rpp29 facilitate catalysis by H1 RNA, which seems to require a phylogenetically conserved pseudoknot structure for function. Unexpectedly, Rpp29 forms a catalytic complex with M1 RNA of E. coli RNase P. The results uncover the core components of eukaryotic RNase P, reveal its evolutionary origin in translation, and provide a paradigm for studying RNA-based catalysis by other nuclear and nucleolar ribonucleoprotein enzymes.

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Eukaryotic RNase P: Role of RNA and protein subunits of a primordial catalytic ribonucleoprotein in RNA-based catalysis

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