Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus

T. Waner*, S. Mazar, E. Nachmias, E. Keren-Kornblatt, S. Harrus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A dot ELISA for the detection of immunoglobulin M (IgM) antibodies to canine distemper virus (CDV) and canine parvovirus (CPV) was assessed. The titres of IgM antibodies to CDV and CPV in 100 dogs were measured by the Immunocomb ELISA kit and compared with the results derived from the immunofluorescene assay (IFA). There was a strong correlation between the results of the dot ELISA technique and the IFA (P<0.001). The dot ELISA kit was also used to assess the changes in the levels of immunoglobulin G (IgG) and IgM antibodies to CPV and CDV in 10 puppies vaccinated with a polyvalent vaccine. High levels of IgM antibodies to CPV were first detected seven days after they were vaccinated, and after nine days all the pups had high titres of IgG antibodies to CPV. High levels of IgM antibodies to CDV were detected after nine days and the highest average titres were recorded after 12 days. IgG antibodies to CDV were present from nine days after vaccination.

Original languageAmerican English
Pages (from-to)588-591
Number of pages4
JournalVeterinary Record
Volume152
Issue number19
DOIs
StatePublished - 10 May 2003

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