Evaluation of a new extrusion device for the production of stable oligolamellar liposomes in a liter scale

Shimon Amselem*, Alberto Gabizon, Yechezkel Barenholz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A procedure for large-scale production of extruded oligolamellar liposomes was developed. the extrusion technique described is rapid, simple, and reproducible. Vesicles with a diameter in the range of 200-500 nm are obtained. the liposomal preparations were characterized by their specific turbidity (determined spectrophotometrically) and contamination with large particles (> 1 μm). Liposome physical stability was determined using a parameter (P) that relates the ratio of the specific turbidity before and after standardized centrifugation. The extruded oligolamellar liposomes obtained were stable, and after 4 weeks storage at 4°C no irreversible aggregation or fusion of the vesicles occurs. The extrusion process ensures sterilization of the final liposomal product, making it acceptable as a pharmaceutical preparation for parenteral use.

Original languageEnglish
Pages (from-to)287-301
Number of pages15
JournalJournal of Liposome Research
Volume1
Issue number3
DOIs
StatePublished - 1989

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