TY - JOUR
T1 - Evaluation of a new extrusion device for the production of stable oligolamellar liposomes in a liter scale
AU - Amselem, Shimon
AU - Gabizon, Alberto
AU - Barenholz, Yechezkel
PY - 1989
Y1 - 1989
N2 - A procedure for large-scale production of extruded oligolamellar liposomes was developed. the extrusion technique described is rapid, simple, and reproducible. Vesicles with a diameter in the range of 200-500 nm are obtained. the liposomal preparations were characterized by their specific turbidity (determined spectrophotometrically) and contamination with large particles (> 1 μm). Liposome physical stability was determined using a parameter (P) that relates the ratio of the specific turbidity before and after standardized centrifugation. The extruded oligolamellar liposomes obtained were stable, and after 4 weeks storage at 4°C no irreversible aggregation or fusion of the vesicles occurs. The extrusion process ensures sterilization of the final liposomal product, making it acceptable as a pharmaceutical preparation for parenteral use.
AB - A procedure for large-scale production of extruded oligolamellar liposomes was developed. the extrusion technique described is rapid, simple, and reproducible. Vesicles with a diameter in the range of 200-500 nm are obtained. the liposomal preparations were characterized by their specific turbidity (determined spectrophotometrically) and contamination with large particles (> 1 μm). Liposome physical stability was determined using a parameter (P) that relates the ratio of the specific turbidity before and after standardized centrifugation. The extruded oligolamellar liposomes obtained were stable, and after 4 weeks storage at 4°C no irreversible aggregation or fusion of the vesicles occurs. The extrusion process ensures sterilization of the final liposomal product, making it acceptable as a pharmaceutical preparation for parenteral use.
UR - http://www.scopus.com/inward/record.url?scp=0024845015&partnerID=8YFLogxK
U2 - 10.3109/08982108909035998
DO - 10.3109/08982108909035998
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0024845015
SN - 0898-2104
VL - 1
SP - 287
EP - 301
JO - Journal of Liposome Research
JF - Journal of Liposome Research
IS - 3
ER -