Evaluation of short-term safety of ultrasound-guided foetal fluid sampling in the dog (Canis lupus familiaris)

Smadar Tal; Gila Kahila Bar-Gal; Sebastian Patrick Arlt

doi:10.1002/vetr.31

Evaluation of short-term safety of ultrasound-guided foetal fluid sampling in the dog (Canis lupus familiaris)

Smadar Tal^*, Gila Kahila Bar-Gal, Sebastian Patrick Arlt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Background: In humans, analysis of amniotic fluid is widely used for diagnostic and prognostic purposes. Amniocentesis has scarcely been used in veterinary medicine to date, despite a tremendous potential for clinical and research applications in dogs. Our study aimed to establish a safe method for foetal fluid sampling in female dogs. Methods: Two transabdominal ultrasound-guided methods were assessed: the “free hand” and the needle-guided bracket sampling. In addition, through a subsequent routinely scheduled ovariohysterectomy, fluid was directly collected. Samples from 98 conceptuses were collected at day 46.7 ± 7.5 of pregnancy. Results: The amount of fluid retrieved varied between 0.5 and 5.0 ml per collection. Macroscopic examination of the uterus and conceptuses identified 53% of the puncture sites. Neither fluid leakage nor foetal injury was detected, and six hematomas (5.8%) were visible. Ultrasound-guided foetal fluid collection was found to be potentially safe, and it can be performed by using either transabdominal method. Conclusion: Foetal fluid collection is possible with relative ease and low short-term risk, and may open paths for diagnostic, therapeutic and research purposes in dogs. The procedure can provide new insights into prenatal clinical medicine, including diagnostics of foetal deaths, early identification of heritable diseases and so on.

Original language	American English
Pages (from-to)	no
Journal	Veterinary Record
Volume	188
Issue number	7
DOIs	https://doi.org/10.1002/vetr.31
State	Published - 1 Apr 2021

Bibliographical note

Funding Information:
Freie Universität Berlin and The Hebrew University of Jerusalem: Joint Seed Money Funding Scheme; Grant Number: HUJI 0366216, 2016.

Funding Information:
information Freie Universit?t Berlin and The Hebrew University of Jerusalem; Joint Seed Money Funding Scheme, Grant/Award Numbers: HUJI0366216,2016 The authors wish to thank the Koret School Veterinary Teaching Hospital for their technical support, as well as Dr. Yehonatan Even Tsur and the staff of the Rishon LeZion municipal veterinary service for their cooperation and participation in this study.

Publisher Copyright:
© 2021 British Veterinary Association

Keywords

allantoic fluid
amniocentesis
amniotic fluid
dog
foetal fluid centesis
pregnancy

Access to Document

10.1002/vetr.31

Cite this

@article{eaabcb2bb4834533ac3c281b93867d19,

title = "Evaluation of short-term safety of ultrasound-guided foetal fluid sampling in the dog (Canis lupus familiaris)",

abstract = "Background: In humans, analysis of amniotic fluid is widely used for diagnostic and prognostic purposes. Amniocentesis has scarcely been used in veterinary medicine to date, despite a tremendous potential for clinical and research applications in dogs. Our study aimed to establish a safe method for foetal fluid sampling in female dogs. Methods: Two transabdominal ultrasound-guided methods were assessed: the “free hand” and the needle-guided bracket sampling. In addition, through a subsequent routinely scheduled ovariohysterectomy, fluid was directly collected. Samples from 98 conceptuses were collected at day 46.7 ± 7.5 of pregnancy. Results: The amount of fluid retrieved varied between 0.5 and 5.0 ml per collection. Macroscopic examination of the uterus and conceptuses identified 53% of the puncture sites. Neither fluid leakage nor foetal injury was detected, and six hematomas (5.8%) were visible. Ultrasound-guided foetal fluid collection was found to be potentially safe, and it can be performed by using either transabdominal method. Conclusion: Foetal fluid collection is possible with relative ease and low short-term risk, and may open paths for diagnostic, therapeutic and research purposes in dogs. The procedure can provide new insights into prenatal clinical medicine, including diagnostics of foetal deaths, early identification of heritable diseases and so on.",

keywords = "allantoic fluid, amniocentesis, amniotic fluid, dog, foetal fluid centesis, pregnancy",

author = "Smadar Tal and Bar-Gal, {Gila Kahila} and Arlt, {Sebastian Patrick}",

note = "Funding Information: Freie Universit{\"a}t Berlin and The Hebrew University of Jerusalem: Joint Seed Money Funding Scheme; Grant Number: HUJI 0366216, 2016. Funding Information: information Freie Universit?t Berlin and The Hebrew University of Jerusalem; Joint Seed Money Funding Scheme, Grant/Award Numbers: HUJI0366216,2016 The authors wish to thank the Koret School Veterinary Teaching Hospital for their technical support, as well as Dr. Yehonatan Even Tsur and the staff of the Rishon LeZion municipal veterinary service for their cooperation and participation in this study. Publisher Copyright: {\textcopyright} 2021 British Veterinary Association",

year = "2021",

month = apr,

day = "1",

doi = "10.1002/vetr.31",

language = "American English",

volume = "188",

pages = "no",

journal = "Veterinary Record",

issn = "0042-4900",

publisher = "British Veterinary Association",

number = "7",

}

TY - JOUR

T1 - Evaluation of short-term safety of ultrasound-guided foetal fluid sampling in the dog (Canis lupus familiaris)

AU - Tal, Smadar

AU - Bar-Gal, Gila Kahila

AU - Arlt, Sebastian Patrick

N1 - Funding Information: Freie Universität Berlin and The Hebrew University of Jerusalem: Joint Seed Money Funding Scheme; Grant Number: HUJI 0366216, 2016. Funding Information: information Freie Universit?t Berlin and The Hebrew University of Jerusalem; Joint Seed Money Funding Scheme, Grant/Award Numbers: HUJI0366216,2016 The authors wish to thank the Koret School Veterinary Teaching Hospital for their technical support, as well as Dr. Yehonatan Even Tsur and the staff of the Rishon LeZion municipal veterinary service for their cooperation and participation in this study. Publisher Copyright: © 2021 British Veterinary Association

PY - 2021/4/1

Y1 - 2021/4/1

N2 - Background: In humans, analysis of amniotic fluid is widely used for diagnostic and prognostic purposes. Amniocentesis has scarcely been used in veterinary medicine to date, despite a tremendous potential for clinical and research applications in dogs. Our study aimed to establish a safe method for foetal fluid sampling in female dogs. Methods: Two transabdominal ultrasound-guided methods were assessed: the “free hand” and the needle-guided bracket sampling. In addition, through a subsequent routinely scheduled ovariohysterectomy, fluid was directly collected. Samples from 98 conceptuses were collected at day 46.7 ± 7.5 of pregnancy. Results: The amount of fluid retrieved varied between 0.5 and 5.0 ml per collection. Macroscopic examination of the uterus and conceptuses identified 53% of the puncture sites. Neither fluid leakage nor foetal injury was detected, and six hematomas (5.8%) were visible. Ultrasound-guided foetal fluid collection was found to be potentially safe, and it can be performed by using either transabdominal method. Conclusion: Foetal fluid collection is possible with relative ease and low short-term risk, and may open paths for diagnostic, therapeutic and research purposes in dogs. The procedure can provide new insights into prenatal clinical medicine, including diagnostics of foetal deaths, early identification of heritable diseases and so on.

AB - Background: In humans, analysis of amniotic fluid is widely used for diagnostic and prognostic purposes. Amniocentesis has scarcely been used in veterinary medicine to date, despite a tremendous potential for clinical and research applications in dogs. Our study aimed to establish a safe method for foetal fluid sampling in female dogs. Methods: Two transabdominal ultrasound-guided methods were assessed: the “free hand” and the needle-guided bracket sampling. In addition, through a subsequent routinely scheduled ovariohysterectomy, fluid was directly collected. Samples from 98 conceptuses were collected at day 46.7 ± 7.5 of pregnancy. Results: The amount of fluid retrieved varied between 0.5 and 5.0 ml per collection. Macroscopic examination of the uterus and conceptuses identified 53% of the puncture sites. Neither fluid leakage nor foetal injury was detected, and six hematomas (5.8%) were visible. Ultrasound-guided foetal fluid collection was found to be potentially safe, and it can be performed by using either transabdominal method. Conclusion: Foetal fluid collection is possible with relative ease and low short-term risk, and may open paths for diagnostic, therapeutic and research purposes in dogs. The procedure can provide new insights into prenatal clinical medicine, including diagnostics of foetal deaths, early identification of heritable diseases and so on.

KW - allantoic fluid

KW - amniocentesis

KW - amniotic fluid

KW - dog

KW - foetal fluid centesis

KW - pregnancy

UR - http://www.scopus.com/inward/record.url?scp=85104135935&partnerID=8YFLogxK

U2 - 10.1002/vetr.31

DO - 10.1002/vetr.31

M3 - Article

C2 - 33835567

AN - SCOPUS:85104135935

SN - 0042-4900

VL - 188

SP - no

JO - Veterinary Record

JF - Veterinary Record

IS - 7

ER -

Evaluation of short-term safety of ultrasound-guided foetal fluid sampling in the dog (Canis lupus familiaris)

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this