TY - JOUR
T1 - Evaluation of some cereals, plants and tubers through protein composition
AU - Gorinstein, Shela
AU - Jaramillo, Nicolas O.
AU - Medina, Oscar J.
AU - Rogriques, Walter A.
AU - Tosello, Geraldo A.
AU - Paredes-Lopez, Octavio
PY - 1999
Y1 - 1999
N2 - Wild and cultivated maize, sorghum, rice, amaranth, soybean, and cassava were screened for variability in seed storage proteins. Total seed proteins, albumin (Alb-1 and Alb-2), globulin, alcohol-soluble (A1 and A2), and glutelin (G1 and G2)fractions were investigated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was done by the obtained protein patterns and their relative amounts. Using quantitative analysis of the protein composition and the electrophoretic patterns, the relationships between total proteins and amount of individual proteins were determined. Electrophoretic patterns of extracted proteins from investigated samples showed that the main protein subunits were concentrated between 10 and 45 kDa. Variation was found in major fractions and minor bands. Electrophoretic patterns of the protein fractions are directly related to the genetic background of the protein and can be identified and used to certify the genetic makeup of wild, cultivated, or newly derived cereals and plants.
AB - Wild and cultivated maize, sorghum, rice, amaranth, soybean, and cassava were screened for variability in seed storage proteins. Total seed proteins, albumin (Alb-1 and Alb-2), globulin, alcohol-soluble (A1 and A2), and glutelin (G1 and G2)fractions were investigated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was done by the obtained protein patterns and their relative amounts. Using quantitative analysis of the protein composition and the electrophoretic patterns, the relationships between total proteins and amount of individual proteins were determined. Electrophoretic patterns of extracted proteins from investigated samples showed that the main protein subunits were concentrated between 10 and 45 kDa. Variation was found in major fractions and minor bands. Electrophoretic patterns of the protein fractions are directly related to the genetic background of the protein and can be identified and used to certify the genetic makeup of wild, cultivated, or newly derived cereals and plants.
KW - Composition
KW - Electrophoresis
KW - Plants
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=53149089116&partnerID=8YFLogxK
U2 - 10.1023/A:1020610424625
DO - 10.1023/A:1020610424625
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C2 - 10609645
AN - SCOPUS:53149089116
SN - 0277-8033
VL - 18
SP - 687
EP - 693
JO - Journal of Protein Chemistry
JF - Journal of Protein Chemistry
IS - 6
ER -