TY - JOUR
T1 - Evolutionary conservation of ribosomal protein mRNA sequences
T2 - application for expansion of corresponding cDNA and gene libraries
AU - Meyuhas, Oded
PY - 1985/8/21
Y1 - 1985/8/21
N2 - Cloned cDNAs, containing ribosomal protein sequences from mouse (five cDNAs) or Xenopus laevis (six cDNAs), were used to estimate the evolutionary conservation, from insects to mammals, of the corresponding mRNA sequences. Northern blot analysis reveals a variable degree of homology between these sequences in different eukaryotes. Thus, among the ribosomal protein cDNA clones utilized, some exhibit complete, others partial, and a few no interphyla cross-hybridization. Melting profile analysis was employed to quantitate this homology. It is proposed that for expansion of eukaryotic ribosomal cDNA and gene libraries, one can exploit the interspecies homology of the corresponding sequences. However, the diverse evolutionary conservation of individual ribosomal protein gene sequences should be taken into account.
AB - Cloned cDNAs, containing ribosomal protein sequences from mouse (five cDNAs) or Xenopus laevis (six cDNAs), were used to estimate the evolutionary conservation, from insects to mammals, of the corresponding mRNA sequences. Northern blot analysis reveals a variable degree of homology between these sequences in different eukaryotes. Thus, among the ribosomal protein cDNA clones utilized, some exhibit complete, others partial, and a few no interphyla cross-hybridization. Melting profile analysis was employed to quantitate this homology. It is proposed that for expansion of eukaryotic ribosomal cDNA and gene libraries, one can exploit the interspecies homology of the corresponding sequences. However, the diverse evolutionary conservation of individual ribosomal protein gene sequences should be taken into account.
KW - Evolutionary conservation
KW - Gene library
KW - mRNA sequence
KW - Nucleic acid hybridization
KW - Ribosomal protein
UR - http://www.scopus.com/inward/record.url?scp=0021858530&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(85)90066-1
DO - 10.1016/0167-4781(85)90066-1
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C2 - 3925998
AN - SCOPUS:0021858530
SN - 0167-4781
VL - 825
SP - 393
EP - 397
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 4
ER -