Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging

Nethanel Friedman; Sharon Merims; Jhonatan Elia; Ofra Benny

doi:10.21769/BioProtoc.4375

Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging

Nethanel Friedman, Sharon Merims, Jhonatan Elia, Ofra Benny^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human skin samples treated with fluorescent nanoparticles. The method is based on horizontal cryosections of skin samples, followed by confocal microscopy and image analysis. This protocol is relatively simple to perform with basic histological tools, thus it can serve for various dermatology assays.

Original language	American English
Article number	e4375
Journal	Bio-protocol
Volume	12
Issue number	7
Early online date	5 Apr 2022
DOIs	https://doi.org/10.21769/BioProtoc.4375
State	Published - 5 Apr 2022

Bibliographical note

Funding Information:
This project was funded by a Kamin fund of the Israel Innovation Authority, and by an Israel Science Foundation (ISF) grant. This protocol served as in our previous work "Physical properties of gold nanoparticles affect skin penetration via hair follicles" (Friedman et al., 2021).

Publisher Copyright:
Copyright © 2022 The Authors.

Keywords

Confocal microscopy
Fluorescent nanoparticles
Horizontal cryosectioning
Nanoparticles penetration depth
Skin permeability

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10.21769/BioProtoc.4375

Cite this

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title = "Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging",

abstract = "Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human skin samples treated with fluorescent nanoparticles. The method is based on horizontal cryosections of skin samples, followed by confocal microscopy and image analysis. This protocol is relatively simple to perform with basic histological tools, thus it can serve for various dermatology assays.",

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AB - Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human skin samples treated with fluorescent nanoparticles. The method is based on horizontal cryosections of skin samples, followed by confocal microscopy and image analysis. This protocol is relatively simple to perform with basic histological tools, thus it can serve for various dermatology assays.

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Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging

Abstract

Bibliographical note

Keywords

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