Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human skin samples treated with fluorescent nanoparticles. The method is based on horizontal cryosections of skin samples, followed by confocal microscopy and image analysis. This protocol is relatively simple to perform with basic histological tools, thus it can serve for various dermatology assays.
Bibliographical noteFunding Information:
This project was funded by a Kamin fund of the Israel Innovation Authority, and by an Israel Science Foundation (ISF) grant. This protocol served as in our previous work "Physical properties of gold nanoparticles affect skin penetration via hair follicles" (Friedman et al., 2021).
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- Confocal microscopy
- Fluorescent nanoparticles
- Horizontal cryosectioning
- Nanoparticles penetration depth
- Skin permeability