Short proteoglycan fragments are of great importance for biochemical research. The solid-phase synthesis of such glycopeptides relies on excessive use of glycosylated amino acids, extended reaction times, and additional post-assembly deprotection protocols. We employed high-shear mixing for expedient and equimolar O-glycopeptide assembly. We further developed a stirring-based deprotection on the solid support, thus completing the synthesis of a glycopeptide library in a minimal amount of time and purification hurdles.
Bibliographical noteFunding Information:
M.H. and D.B.A.A are supported by COST Action 18103 “INNOGLY” and European Innovation Council project “Smellodi” funded by the European Union.
© 2022 The Authors. European Journal of Organic Chemistry published by Wiley-VCH GmbH.
- Glycosylated amino acids
- Solid-phase synthesis
- Synthetic methods