Expression and assembly of the potato virus Y (PVY) coat protein (CP) in Escherichia coli cells

Yehuda Stram*, Ilan Sela, Orit Edelbaum, Edna Tanne, Miri Karchi, Hagai Karchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A clone harboring the full-length cDNA of potato virus Y in a lambda-DASH vector under the control of a T7 promoter was introduced into Escherichia coli carrying the T7-RNA-polymerase gene on a plasmid. The viral coat protein was expressed and the product was of the same size as the corresponding mature protein in infected plants. Immunoelectronmicroscopy of transfected cell extracts revealed virus-like particles, indicating that the proteins involved in its processing and the viral coat protein retained their native activity.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalVirus Research
Volume28
Issue number1
DOIs
StatePublished - Apr 1993

Keywords

  • Assembly
  • Coat protein
  • PVY
  • T7 promoter/polymerase

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