Abstract
A clone harboring the full-length cDNA of potato virus Y in a lambda-DASH vector under the control of a T7 promoter was introduced into Escherichia coli carrying the T7-RNA-polymerase gene on a plasmid. The viral coat protein was expressed and the product was of the same size as the corresponding mature protein in infected plants. Immunoelectronmicroscopy of transfected cell extracts revealed virus-like particles, indicating that the proteins involved in its processing and the viral coat protein retained their native activity.
Original language | English |
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Pages (from-to) | 29-35 |
Number of pages | 7 |
Journal | Virus Research |
Volume | 28 |
Issue number | 1 |
DOIs | |
State | Published - Apr 1993 |
Keywords
- Assembly
- Coat protein
- PVY
- T7 promoter/polymerase