Expression and localization of muscarinic receptors in P19-derived neurons

Dorit Parnas; Eliahu Heldman; Leora Branski; Naomi Feinstein; Michal Linial

doi:10.1007/BF02737082

Expression and localization of muscarinic receptors in P19-derived neurons

Dorit Parnas, Eliahu Heldman, Leora Branski, Naomi Feinstein, Michal Linial^*

^*Corresponding author for this work

Department of Biological Chemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.

Original language	English
Pages (from-to)	17-29
Number of pages	13
Journal	Journal of Molecular Neuroscience
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF02737082
State	Published - 1998

Bibliographical note

Funding Information:
We would like to thank A. I. Levey (Emory University, Atlanta) for the generous gifts of antibodies. We also thank the Smith laboratory in the Hebrew University for the use of their facilities. M. L. is a member of the Otto Lewie Minerva Center for molecular and cellular neurobiology in Jerusalem. This work was partially supported by the National Institute for Psychobiology in Israel.

Keywords

Adenylyl cyclase
Embryonal carcinoma
Inositol (1,4,5)- trisphosphate
Neuronal cell-line
Presynaptic receptors

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10.1007/BF02737082

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abstract = "The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.",

keywords = "Adenylyl cyclase, Embryonal carcinoma, Inositol (1,4,5)- trisphosphate, Neuronal cell-line, Presynaptic receptors",

author = "Dorit Parnas and Eliahu Heldman and Leora Branski and Naomi Feinstein and Michal Linial",

note = "Funding Information: We would like to thank A. I. Levey (Emory University, Atlanta) for the generous gifts of antibodies. We also thank the Smith laboratory in the Hebrew University for the use of their facilities. M. L. is a member of the Otto Lewie Minerva Center for molecular and cellular neurobiology in Jerusalem. This work was partially supported by the National Institute for Psychobiology in Israel.",

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AU - Heldman, Eliahu

AU - Branski, Leora

AU - Feinstein, Naomi

AU - Linial, Michal

N1 - Funding Information: We would like to thank A. I. Levey (Emory University, Atlanta) for the generous gifts of antibodies. We also thank the Smith laboratory in the Hebrew University for the use of their facilities. M. L. is a member of the Otto Lewie Minerva Center for molecular and cellular neurobiology in Jerusalem. This work was partially supported by the National Institute for Psychobiology in Israel.

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N2 - The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.

AB - The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.

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Expression and localization of muscarinic receptors in P19-derived neurons

Abstract

Bibliographical note

Keywords

UN SDGs

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