Expression and secretion of proteins in E. coli

Ophry Pines*, Masayori Inouye

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

47 Scopus citations


This review outlines approaches to the cloning and expression of proteins in Escherichia coli. The expression vectors described here (pIN-III derivatives) utilize the strong lipoprotein promoter, which is controlled by the lac-UV5 promoter-operator. These vectors provide the means for targeting a protein to any of the four subcellular compartments of the bacterial cell: cytoplasm, cytoplasmic membrane, periplasm, and outer membrane. Of particular importance is that secretion of proteins into the E. coli periplasm (using the OmpA signal peptide) is applicable for the production of both prokaryotic and eukaryotic proteins thereby enhancing protein activity and stability.

Original languageAmerican English
Pages (from-to)25-34
Number of pages10
JournalMolecular Biotechnology
Issue number1
StatePublished - 1999


  • E. coli
  • Eukaryote
  • Expression vectors
  • OmpA
  • Prokaryote
  • Secretion
  • pIN-III


Dive into the research topics of 'Expression and secretion of proteins in E. coli'. Together they form a unique fingerprint.

Cite this