TY - JOUR
T1 - Expression of attaching/effacing activity by enteropathogenic Escherichia coli depends on growth phase, temperature, and protein synthesis upon contact with epithelial cells
AU - Rosenshine, Ilan
AU - Ruschkowski, Sharon
AU - Brett Finlay, B.
PY - 1996
Y1 - 1996
N2 - Enteropathogenic Escherichia coli (EPEC) inducts tyrosine phosphorylation of a 90-kDa protein (Hp90) in infected epithelial cells. This in turn facilitates intimate binding of EPEC via the outer membrane protein intimin, effacement of host cell microvilli, cytoskeletal rearrangement, and bacterial uptake. This phenotype has been commonly referred to as attaching/effacing (A/E). The ability of EPEC to induce A/E lesions was dependent on bacterial growth phase and temperature. Early-logarithmic-phase EPEC grown at 37°C elicits strong A/E activity within minutes after infection of HeLa epithelial cells. EPEC de novo protein synthesis during the first minutes of interaction with the host cell was required to elicit A/E lesions. However, once formed, bacterial viability was not needed to maintain A/E lesions. The type of growth media and partial O2 pressure level do not seem to affect the ability of EPEC to cause A/E lesions. These results indicates that the A/E activity of EPEC is tightly regulated by environmental and host factors.
AB - Enteropathogenic Escherichia coli (EPEC) inducts tyrosine phosphorylation of a 90-kDa protein (Hp90) in infected epithelial cells. This in turn facilitates intimate binding of EPEC via the outer membrane protein intimin, effacement of host cell microvilli, cytoskeletal rearrangement, and bacterial uptake. This phenotype has been commonly referred to as attaching/effacing (A/E). The ability of EPEC to induce A/E lesions was dependent on bacterial growth phase and temperature. Early-logarithmic-phase EPEC grown at 37°C elicits strong A/E activity within minutes after infection of HeLa epithelial cells. EPEC de novo protein synthesis during the first minutes of interaction with the host cell was required to elicit A/E lesions. However, once formed, bacterial viability was not needed to maintain A/E lesions. The type of growth media and partial O2 pressure level do not seem to affect the ability of EPEC to cause A/E lesions. These results indicates that the A/E activity of EPEC is tightly regulated by environmental and host factors.
UR - http://www.scopus.com/inward/record.url?scp=0030067440&partnerID=8YFLogxK
U2 - 10.1128/iai.64.3.966-973.1996
DO - 10.1128/iai.64.3.966-973.1996
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C2 - 8641808
AN - SCOPUS:0030067440
SN - 0019-9567
VL - 64
SP - 966
EP - 973
JO - Infection and Immunity
JF - Infection and Immunity
IS - 3
ER -