TY - JOUR
T1 - Expression of Chromosomally InsertedBacillus thuringiensis israelensisToxin Genes inBacillus sphaericus
AU - Bar, E.
AU - Sandler, N.
AU - Makayoto, M.
AU - Keynan, A.
PY - 1998/11
Y1 - 1998/11
N2 - Bacillus thuringiensis israelensisδ-endotoxin genes were inserted into transposon Tn917in plasmid pTV51Ts and cloned into the chromosome ofBacillus sphaericus2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDaB.t.israelensistoxin proteins and were approximately 10 times more toxic toA. aegyptilarvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-typeB. sphaericus.The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure.
AB - Bacillus thuringiensis israelensisδ-endotoxin genes were inserted into transposon Tn917in plasmid pTV51Ts and cloned into the chromosome ofBacillus sphaericus2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDaB.t.israelensistoxin proteins and were approximately 10 times more toxic toA. aegyptilarvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-typeB. sphaericus.The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure.
UR - http://www.scopus.com/inward/record.url?scp=0000533834&partnerID=8YFLogxK
U2 - 10.1006/jipa.1998.4787
DO - 10.1006/jipa.1998.4787
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AN - SCOPUS:0000533834
SN - 0022-2011
VL - 72
SP - 206
EP - 213
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 3
ER -