Expression of Chromosomally InsertedBacillus thuringiensis israelensisToxin Genes inBacillus sphaericus

E. Bar*, N. Sandler, M. Makayoto, A. Keynan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Bacillus thuringiensis israelensisδ-endotoxin genes were inserted into transposon Tn917in plasmid pTV51Ts and cloned into the chromosome ofBacillus sphaericus2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDaB.t.israelensistoxin proteins and were approximately 10 times more toxic toA. aegyptilarvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-typeB. sphaericus.The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure.

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalJournal of Invertebrate Pathology
Volume72
Issue number3
DOIs
StatePublished - Nov 1998

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