Expression of endo-1,4-β-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening

Ziv Shani, Mara Dekel, Levava Roiz, Miri Horowitz, Natalia Kolosovski, Shaul Lapidot, Sari Alkan, Hinanit Koltai, Galit Tsabary, Raphael Goren, Oded Shoseyov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Arabidopsis thaliana CEL1 protein was detected in young expanding tissues. Immunostaining revealed that CEL1 accumulated mostly in xylem cells. The primary, as well as the secondary xylem showed considerable CEL1 staining. CEL1 was also observed in young epidermal cells, in which the thicker lateral and tangential walls stained more intensely than the inner walls. In newly formed cell walls, the lateral tangential walls were labeled more intensively than the inner walls. Cellulase activity was found to be significantly higher in growing tissue compared to mature parts of the plant. Cel1 expression concurrently with cellulase activity could be restored in detached matured leaves by sucrose treatment after 48 h in the culture medium.

Original languageAmerican English
Pages (from-to)1067-1074
Number of pages8
JournalPlant Cell Reports
Volume25
Issue number10
DOIs
StatePublished - Oct 2006

Keywords

  • Cell wall
  • Cellulase
  • Endoglucanase
  • Xylem development

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