Expression of matrix metalloproteinases during impairment and recovery of the avian growth plate

H. Dan, S. Simsa-Maziel, A. Hisdai, D. Sela-Donenfeld, E. Monsonego Ornan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Tibial dyschondroplasia (TD) is a prevalent skeletal abnormality associated with rapid growth rate in many avian species. It is characterized by the presence of a nonvascularized, nonmineralized lesion that extends from the epiphyseal growth plate into the metaphysis of the proximal tibiotarsal bones. In this study, we examined the expression of 4 members of the matrix metalloproteinase (MMP) family (MMP-2, -3, -9, and -13) in thiram-induced TD lesions and in the process of recovery from TD, by in situ hybridization analysis and quantitative real-time PCR. A model for the induction and recovery of TD was established, consisting of 3 groups of broilers: (1) thiram group, chicks fed a thiram-enriched diet to induce TD; (2) recovery group, chicks fed a thiram-enriched diet during the first week of the experiment and a normal diet from the sec-ond week on; and (3) control group, chicks fed a normal diet throughout the experimental period. In agreement with our previous data, the 4 MMP were diminished in the TD lesion (P < 0.05); however, in the current study we show that the growth plate was able to repair itself and that the MMP reappeared during the process of recovery from TD. Our results strengthen the link be-tween MMP expression and growth-plate impairment, and we suggest that gelatinase activity (MMP-2 and 9) facilitates this process.

Original languageAmerican English
Pages (from-to)3544-3555
Number of pages12
JournalJournal of Animal Science
Volume87
Issue number11
DOIs
StatePublished - Nov 2009

Keywords

  • Avian
  • Growth plate
  • Matrix metalloproteinase
  • Tibial dyschondroplasia

Fingerprint

Dive into the research topics of 'Expression of matrix metalloproteinases during impairment and recovery of the avian growth plate'. Together they form a unique fingerprint.

Cite this