Expression of mRNA of Lipoprotein Receptor Related Protein 8, Low Density Lipoprotein Receptor, and Very Low Density Lipoprotein Receptor in Bovine Ovarian Cells During Follicular Development and Corpus Luteum Formation and Regression

Nurit Argov, David Sklan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Lipoproteins in the plasma are the major source of cholesterol obtained by the ovarian theca and granulosa cells for steroidogenesis. In this study, we have identified mRNA expression in bovine theca and granulosa cells of two lipoprotein receptors, low density lipoprotein receptor (LDLr-) and very low density lipoprotein receptor (VLDLr) in granulosa cells from small antral follicles through preovulatory follicles and in theca cells from large and medium sized antral follicles. In the corpus luteum (CL) both these receptors were found in the developing and differentiating stages whereas only mRNA for VLDLr was detected in the regression stage. This study also described for the first time, the presence of lipoprotein receptor related protein (LRP8) in granulosa cells from small antral follicles through preovulatory follicles and in theca cells from large and medium sized antral follicles. This may indicate a role of LRP8 in cholesterol delivery to steriodogenic cells. LRP8 was not detected in any of the CL stages. The roles of the LDLr superfamily in lipid transport to ovarian cells and its participation in follicular and CL development and regression is discussed.

Original languageAmerican English
Pages (from-to)169-175
Number of pages7
JournalMolecular Reproduction and Development
Volume68
Issue number2
DOIs
StatePublished - Jun 2004

Keywords

  • Follicular development
  • Granulosa cells
  • Ovulatory cycle
  • Steroid hormones
  • Theca cells

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