Expression of prostaglandin F (PGF ) receptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF -induced luteolysis

K. Shirasuna, Y. Akabane, N. Beindorff, K. Nagai, M. Sasaki, T. Shimizu, H. Bollwein, R. Meidan, A. Miyamoto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Prostaglandin F (PGF ) induces luteolysis via a specific receptor, PTGFR. Although PTGFR mRNA expression in the bovine corpus luteum (CL) has been studied previously, changes in PTGFR protein and its localization are not fully understood during the life span of the CL. In addition to full-length PTGFR, several types of PTGFR isoforms, such as PTGFRα (type I) and PTGFRζ (type II), were reported in the bovine CL, suggesting isoform-specific luteal action. Full-length PTGFR mRNA in the bovine CL increased from the early to the mid-luteal phase and decreased during luteolysis, whereas PTGFR protein remained stable. PTGFR protein was localized to both luteal and endothelial cells and was expressed similarly during the life span of the CL. Like full-length PTGFR mRNA, PTGFRα and PTGFRζ mRNA also increased from the early to mid-luteal phases, and mRNA of PTGFRζ, but not PTGFRα, decreased in the regressing CL. During PGF -induced luteolysis, the mRNAs of full-length PTGFR, PTGFR,α and PTGFRζ decreased rapidly (from 5 or 15 min after PGF injection), but PTGFR protein decreased only 12 h later. Silencing full-length PTGFR using small interfering RNA prevented PGF -stimulated cyclooxygenase-2 (PTGS2) mRNA induction. By contrast, PGF could stimulate vascular endothelial growth factor A (VEGFA) mRNA even when full-length PTGFR was knocked down, thus suggesting that PGF may stimulate PTGS2 via full-length PTGFR, whereas VEGFA is stimulated via other PTGFR isoforms. Collectively, PTGFR protein was expressed continually in the bovine CL during the estrous cycle, implying that PGF could function throughout this period. Additionally, the bovine CL expresses different PTGFR isoforms, and thus PGF may have different effects when acting via full-length PTGFR or via PTGFR isoforms.

Original languageEnglish
Pages (from-to)227-238
Number of pages12
JournalDomestic Animal Endocrinology
Volume43
Issue number3
DOIs
StatePublished - Oct 2012

Keywords

  • Corpus luteum
  • Cow
  • Luteolysis
  • PTGFR

Fingerprint

Dive into the research topics of 'Expression of prostaglandin F (PGF ) receptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF -induced luteolysis'. Together they form a unique fingerprint.

Cite this