We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.
|Original language||American English|
|Number of pages||10|
|Journal||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology|
|State||Published - 1 Jan 2003|
Bibliographical noteFunding Information:
Thanks are due to Dr David J. Hartshorne (Muscle Biology Group, University of Arizona, USA) for the human PP1δ protein. Our work was supported by the grants of the Hungarian Ministry of Education (FKFP 100/2000), the National Research Found (OTKA T33050) and by a bilateral Hungarian–Israeli exchange program (ISR-2/96).
- Developmental analysis
- Gene cloning
- Gene localization
- Neurospora crassa
- Phosphoprotein phosphatase 1
- Protein dephosphorylation