Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

Tamás Zeke; Endre Kókai; Balázs Szöor; Einat Yatzkan; Oded Yarden; Krisztina Szirák; Zsigmond Fehér; Péter Bagossi; Pál Gergely; Viktor Dombrádi

doi:10.1016/S1096-4959(02)00188-4

Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

Tamás Zeke, Endre Kókai, Balázs Szöor, Einat Yatzkan, Oded Yarden, Krisztina Szirák, Zsigmond Fehér, Péter Bagossi, Pál Gergely, Viktor Dombrádi^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.

Original language	English
Pages (from-to)	161-170
Number of pages	10
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/S1096-4959(02)00188-4
State	Published - 1 Jan 2003

Bibliographical note

Funding Information:
Thanks are due to Dr David J. Hartshorne (Muscle Biology Group, University of Arizona, USA) for the human PP1δ protein. Our work was supported by the grants of the Hungarian Ministry of Education (FKFP 100/2000), the National Research Found (OTKA T33050) and by a bilateral Hungarian–Israeli exchange program (ISR-2/96).

Keywords

Developmental analysis
Gene cloning
Gene localization
Neurospora crassa
Phosphoprotein phosphatase 1
Protein dephosphorylation

Access to Document

10.1016/S1096-4959(02)00188-4

Cite this

@article{07a8b7a49cd44ea1ada230143cc03a53,

title = "Expression of protein phosphatase 1 during the asexual development of Neurospora crassa",

abstract = "We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.",

keywords = "Developmental analysis, Gene cloning, Gene localization, Neurospora crassa, Phosphoprotein phosphatase 1, Protein dephosphorylation",

author = "Tam{\'a}s Zeke and Endre K{\'o}kai and Bal{\'a}zs Sz{\"o}or and Einat Yatzkan and Oded Yarden and Krisztina Szir{\'a}k and Zsigmond Feh{\'e}r and P{\'e}ter Bagossi and P{\'a}l Gergely and Viktor Dombr{\'a}di",

note = "Funding Information: Thanks are due to Dr David J. Hartshorne (Muscle Biology Group, University of Arizona, USA) for the human PP1δ protein. Our work was supported by the grants of the Hungarian Ministry of Education (FKFP 100/2000), the National Research Found (OTKA T33050) and by a bilateral Hungarian–Israeli exchange program (ISR-2/96).",

year = "2003",

month = jan,

day = "1",

doi = "10.1016/S1096-4959(02)00188-4",

language = "אנגלית",

volume = "134",

pages = "161--170",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

AU - Zeke, Tamás

AU - Kókai, Endre

AU - Szöor, Balázs

AU - Yatzkan, Einat

AU - Yarden, Oded

AU - Szirák, Krisztina

AU - Fehér, Zsigmond

AU - Bagossi, Péter

AU - Gergely, Pál

AU - Dombrádi, Viktor

N1 - Funding Information: Thanks are due to Dr David J. Hartshorne (Muscle Biology Group, University of Arizona, USA) for the human PP1δ protein. Our work was supported by the grants of the Hungarian Ministry of Education (FKFP 100/2000), the National Research Found (OTKA T33050) and by a bilateral Hungarian–Israeli exchange program (ISR-2/96).

PY - 2003/1/1

Y1 - 2003/1/1

N2 - We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.

AB - We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.

KW - Developmental analysis

KW - Gene cloning

KW - Gene localization

KW - Neurospora crassa

KW - Phosphoprotein phosphatase 1

KW - Protein dephosphorylation

UR - http://www.scopus.com/inward/record.url?scp=0037229497&partnerID=8YFLogxK

U2 - 10.1016/S1096-4959(02)00188-4

DO - 10.1016/S1096-4959(02)00188-4

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 12524044

AN - SCOPUS:0037229497

SN - 1096-4959

VL - 134

SP - 161

EP - 170

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 1

ER -

Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this