Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves

D Guenoune; R Amir; S Wolf; S Wininger; H Badani; B Ben-Dor; S Galili

Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves

D Guenoune, R Amir, S Wolf, S Wininger, H Badani, B Ben-Dor, S Galili

Institute of Plant Sciences and Genetics in Agriculture

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Vegetative storage proteins (VSPs) were originally identified as leaf proteins that accumulate to about 45% of the total soluble leaf proteins in depodded soybean plants (Staswick, 1990). In this work we expressed soybean VSPα in transgenic tobacco plants under the control of CaMV 35S promoter. Analysis by SDS Polyacrylamide gels revealed a similar molecular weight for the soybean VSPα in transgenic and in wild type (WT) plants. This observation suggests that soybean VSPα in transgenic plants follows corrected processing. We infer that tobacco plants provide a suitable system for studying post-transcriptional and post translation regulation of VSP.

Original language	American English
Title of host publication	Plant Biotechnology and In Vitro Biology in the 21st Century
Subtitle of host publication	Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998
Editors	Arie Altman, Meira Ziv, Izhar Shamay
Place of Publication	Dordrecht, Boston
Publisher	Springer
Pages	293-296
Number of pages	4
Volume	36
ISBN (Electronic)	9789401146616
ISBN (Print)	9789401146616, 9401146616
State	Published - 1999

Publication series

Name	Current Plant Science and Biotechnology in Agriculture
Publisher	Springer Science & Business Media
Volume	36

Access to Document

https://link.springer.com/chapter/10.1007/978-94-011-4661-6_68

Cite this

Guenoune, D., Amir, R., Wolf, S., Wininger, S., Badani, H., Ben-Dor, B., & Galili, S. (1999). Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves. In A. Altman, M. Ziv, & I. Shamay (Eds.), Plant Biotechnology and In Vitro Biology in the 21st Century: Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998 (Vol. 36, pp. 293-296). (Current Plant Science and Biotechnology in Agriculture; Vol. 36). Springer. https://link.springer.com/chapter/10.1007/978-94-011-4661-6_68

Guenoune, D ; Amir, R ; Wolf, S et al. / Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves. Plant Biotechnology and In Vitro Biology in the 21st Century: Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998. editor / Arie Altman ; Meira Ziv ; Izhar Shamay. Vol. 36 Dordrecht, Boston : Springer, 1999. pp. 293-296 (Current Plant Science and Biotechnology in Agriculture).

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title = "Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves",

abstract = "Vegetative storage proteins (VSPs) were originally identified as leaf proteins that accumulate to about 45% of the total soluble leaf proteins in depodded soybean plants (Staswick, 1990). In this work we expressed soybean VSPα in transgenic tobacco plants under the control of CaMV 35S promoter. Analysis by SDS Polyacrylamide gels revealed a similar molecular weight for the soybean VSPα in transgenic and in wild type (WT) plants. This observation suggests that soybean VSPα in transgenic plants follows corrected processing. We infer that tobacco plants provide a suitable system for studying post-transcriptional and post translation regulation of VSP.",

author = "D Guenoune and R Amir and S Wolf and S Wininger and H Badani and B Ben-Dor and S Galili",

year = "1999",

language = "American English",

isbn = "9789401146616",

volume = "36",

series = "Current Plant Science and Biotechnology in Agriculture",

publisher = "Springer",

pages = "293--296",

editor = "Arie Altman and Ziv, {Meira } and Izhar Shamay",

booktitle = "Plant Biotechnology and In Vitro Biology in the 21st Century",

}

Guenoune, D, Amir, R, Wolf, S, Wininger, S, Badani, H, Ben-Dor, B & Galili, S 1999, Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves. in A Altman, M Ziv & I Shamay (eds), Plant Biotechnology and In Vitro Biology in the 21st Century: Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998. vol. 36, Current Plant Science and Biotechnology in Agriculture, vol. 36, Springer, Dordrecht, Boston, pp. 293-296. <https://link.springer.com/chapter/10.1007/978-94-011-4661-6_68>

Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves. / Guenoune, D; Amir, R; Wolf, S et al.
Plant Biotechnology and In Vitro Biology in the 21st Century: Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998. ed. / Arie Altman; Meira Ziv; Izhar Shamay. Vol. 36 Dordrecht, Boston: Springer, 1999. p. 293-296 (Current Plant Science and Biotechnology in Agriculture; Vol. 36).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves

AU - Guenoune, D

AU - Amir, R

AU - Wolf, S

AU - Wininger, S

AU - Badani, H

AU - Ben-Dor, B

AU - Galili, S

PY - 1999

Y1 - 1999

N2 - Vegetative storage proteins (VSPs) were originally identified as leaf proteins that accumulate to about 45% of the total soluble leaf proteins in depodded soybean plants (Staswick, 1990). In this work we expressed soybean VSPα in transgenic tobacco plants under the control of CaMV 35S promoter. Analysis by SDS Polyacrylamide gels revealed a similar molecular weight for the soybean VSPα in transgenic and in wild type (WT) plants. This observation suggests that soybean VSPα in transgenic plants follows corrected processing. We infer that tobacco plants provide a suitable system for studying post-transcriptional and post translation regulation of VSP.

AB - Vegetative storage proteins (VSPs) were originally identified as leaf proteins that accumulate to about 45% of the total soluble leaf proteins in depodded soybean plants (Staswick, 1990). In this work we expressed soybean VSPα in transgenic tobacco plants under the control of CaMV 35S promoter. Analysis by SDS Polyacrylamide gels revealed a similar molecular weight for the soybean VSPα in transgenic and in wild type (WT) plants. This observation suggests that soybean VSPα in transgenic plants follows corrected processing. We infer that tobacco plants provide a suitable system for studying post-transcriptional and post translation regulation of VSP.

M3 - Chapter

SN - 9789401146616

SN - 9401146616

VL - 36

T3 - Current Plant Science and Biotechnology in Agriculture

SP - 293

EP - 296

BT - Plant Biotechnology and In Vitro Biology in the 21st Century

A2 - Altman, Arie

A2 - Ziv, Meira

A2 - Shamay, Izhar

PB - Springer

CY - Dordrecht, Boston

ER -

Guenoune D, Amir R, Wolf S, Wininger S, Badani H, Ben-Dor B et al. Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves. In Altman A, Ziv M, Shamay I, editors, Plant Biotechnology and In Vitro Biology in the 21st Century: Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology Jerusalem, Israel, 14–19 June 1998. Vol. 36. Dordrecht, Boston: Springer. 1999. p. 293-296. (Current Plant Science and Biotechnology in Agriculture).

Expression of Soybean Vegetative Storage Proteins (VSP$) in Tobacco Leaves

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