Expression of the "helper component" protein of potato virus Y (PVY) in E. coli: Possible involvement of a third protease

Yehuda Stram*, Aviva Chetsrony, Hagai Karchi, Miri Karchi, Orit Edelbaum, Eyal Vardi, Orna Livneh, Ilan Sela

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Transmission of potyviruses by aphids depends on the presence of a virus encoded helper-component protein (HC) that also exhibits protease activity. HC was expressed in E. coli from two types of clones: a full-length cDNA clone of PVY and two 5′ end clones containing the first three cistrons (3.6-3.7 kbp). The clones derived from the 5′ end of PVY expressed HC of the size of the mature component. Other proteins reacting with antibodies to HC were also observed, and their sizes corresponded with those of expected intermediates resulting from partial protease cleavage of the three-cistron polyprotein. On the other hand, the only detectable HC-related product of the full-length clone was a mature-size HC. The presence of a third PVY protease among the first three cistrons is therefore suggested.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalVirus Genes
Volume7
Issue number2
DOIs
StatePublished - Jun 1993

Keywords

  • helper component
  • protease
  • PVY

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