The spatial pattern of expression of the murine homeo box-containing gene Hox 1.5 was studied during embryogenesis. In situ hybridization of single-stranded RNA probes to mouse embryo sections revealed a specific spatial distribution of the Hox 1.5 transcripts in mouse embryos 8.5 to 12.5 days postcoitum (p.c.). Analysis of mouse embryos 8.5 days p.c. showed that the gene is expressed in a spatially restricted manner. Expression appears to be limited to the central nervous system with an anterior boundary in the hindbrain and extending posteriorly through caudal regions of the spinal cord. The same spatial pattern of expression was observed in embryos 9.5 to 12.5 days p.c. These results show that the murine Hox 1.5 gene is expressed in a spatially restricted manner during embryonic development similar to the patterns observed in Drosophila homeotic genes.
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We thank Allen Fienberg, Manuel Utset, and Dr. William McGinnis for helpful discussions and critical reading of the manuscript. We also thank Dr. Martha Constantine-Paton for her helpful comments and the use of her equipment and Dr. Janet Rossant and Dr. Virginia Papaiannou for their help in the interpretation of the results. A.F. is a National Cancer Cytology Center Postdoctoral Fellow. This work was supported in part by National Institutes of Health Grant GM-09966 to F.H.R. and a gift from an anonymous donor to F.H.R.