TY - JOUR
T1 - Expression of the novel wheat gene TM20 confers enhanced cadmium tolerance to bakers' yeast
AU - Kim, Yu Young
AU - Kim, Do Young
AU - Shim, Donghwan
AU - Song, Won Yong
AU - Lee, Joohyun
AU - Schroeder, Julian I.
AU - Kim, Sanguk
AU - Moran, Nava
AU - Lee, Youngsook
PY - 2008/6/6
Y1 - 2008/6/6
N2 - Cadmium causes the generation of reactive oxygen species, which in turn causes cell damage. We isolated a novel gene from a wheat root cDNA library, which conferred Cd(II)-specific tolerance when expressed in yeast (Saccharomyces cerevisiae). The gene, which we called TaTM20, for Triticum aestivum transmembrane 20, encodes a putative hydrophobic polypeptide of 889 amino acids, containing 20 transmembrane domains arranged as a 5-fold internal repeating unit of 4 transmembrane domains each. Expression of TaTM20 in yeast cells stimulated Cd(II) efflux resulting in a decrease in the content of yeast intracellular cadmium. TaTM20-induced Cd(II) tolerance was maintained in yeast even under conditions of reduced GSH. These results demonstrate that TaTM20 enhances Cd(II) tolerance in yeast through the stimulation of Cd(II) efflux from the cell, partially independent of GSH. Treatment of wheat seedlings with Cd(II) induced their expression of TaTM20, decreasing subsequent root Cd(II) accumulation and suggesting a possible role for TaTM20 in Cd(II) tolerance in wheat.
AB - Cadmium causes the generation of reactive oxygen species, which in turn causes cell damage. We isolated a novel gene from a wheat root cDNA library, which conferred Cd(II)-specific tolerance when expressed in yeast (Saccharomyces cerevisiae). The gene, which we called TaTM20, for Triticum aestivum transmembrane 20, encodes a putative hydrophobic polypeptide of 889 amino acids, containing 20 transmembrane domains arranged as a 5-fold internal repeating unit of 4 transmembrane domains each. Expression of TaTM20 in yeast cells stimulated Cd(II) efflux resulting in a decrease in the content of yeast intracellular cadmium. TaTM20-induced Cd(II) tolerance was maintained in yeast even under conditions of reduced GSH. These results demonstrate that TaTM20 enhances Cd(II) tolerance in yeast through the stimulation of Cd(II) efflux from the cell, partially independent of GSH. Treatment of wheat seedlings with Cd(II) induced their expression of TaTM20, decreasing subsequent root Cd(II) accumulation and suggesting a possible role for TaTM20 in Cd(II) tolerance in wheat.
UR - http://www.scopus.com/inward/record.url?scp=47049088153&partnerID=8YFLogxK
U2 - 10.1074/jbc.M708947200
DO - 10.1074/jbc.M708947200
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C2 - 18411273
AN - SCOPUS:47049088153
SN - 0021-9258
VL - 283
SP - 15893
EP - 15902
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 23
ER -