Expression of the two cytochrome P450 aromatase genes in the male and female blue gourami (Trichogaster trichopterus) during the reproductive cycle

Meital Ezagouri, Svetlana Yom-Din, Doron Goldberg, Karen Jackson, Berta Levavi-Sivan, Gad Degani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In this study, the involvement of the cytochrome P450 aromatase gene (CYP19) in the gametogenesis of the teleost blue gourami (Trichogaster trichopterus) is described. The blue gourami brain CYP19 (bgCYP19b) and gonadal CYP19 (bgCYP19a) aromatase genes were cloned and their expression analyzed during the different reproductive stages. The cloned cDNAs of the bgCYP19b and bgCYP19a were found to contain segments of 1518 bp (an open reading frame encoding a deduced protein of 506 residues) and 489 bp (encoding a peptide of 163 residues), respectively. Although the mRNA levels of bgCYP19b were very low in females until the vitellogenic phase, they were significantly higher in the final oocyte maturation stage. The aromatase gene mRNA levels in the gonads were significantly lower in females in the high vitellogenic stage, as compared to females during early vitellogenesis or maturation. In males, the mRNA levels of bgCYP19b were significantly lower in juveniles than in mature individuals. However, no significant differences were observed between mature non-reproductive and reproductive males. In addition, there was no significant difference between the expression of bgCYP19a in juvenile and non-nest building mature males, although a significant increase was detected in mature reproductive males. Although CYP19b expression was similar in both sexes, the expression of CYP19a was significantly different between males and females.

Original languageAmerican English
Pages (from-to)208-213
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume159
Issue number2-3
DOIs
StatePublished - 2008

Keywords

  • Aromatase
  • CYP19
  • Gonadal cycle
  • Gourami
  • Reproduction

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