TY - JOUR
T1 - Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)
AU - Unge, Torsten
AU - Ahola, Harri
AU - Bhikhabhai, Ramagauri
AU - Bäckbro, Kristina
AU - Lövgren, Seved
AU - Strandberg, Bror
AU - Fenyö, Eva Maria
AU - Honigman, Alik
AU - Panet, Amos
AU - Gronowitz, J. Simon
PY - 1990/11
Y1 - 1990/11
N2 - The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
AB - The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
UR - http://www.scopus.com/inward/record.url?scp=0025224522&partnerID=8YFLogxK
U2 - 10.1089/aid.1990.6.1297
DO - 10.1089/aid.1990.6.1297
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C2 - 1706608
AN - SCOPUS:0025224522
SN - 0889-2229
VL - 6
SP - 1297
EP - 1303
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 11
ER -