Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)

Torsten Unge*, Harri Ahola, Ramagauri Bhikhabhai, Kristina Bäckbro, Seved Lövgren, Bror Strandberg, Eva Maria Fenyö, Alik Honigman, Amos Panet, J. Simon Gronowitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.

Original languageEnglish
Pages (from-to)1297-1303
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume6
Issue number11
DOIs
StatePublished - Nov 1990

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