Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)

Torsten Unge; Harri Ahola; Ramagauri Bhikhabhai; Kristina Bäckbro; Seved Lövgren; Bror Strandberg; Eva Maria Fenyö; Alik Honigman; Amos Panet; J. Simon Gronowitz

doi:10.1089/aid.1990.6.1297

Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)

Torsten Unge^*
, Harri Ahola
, Ramagauri Bhikhabhai
, Kristina Bäckbro
, Seved Lövgren
, Bror Strandberg
, Eva Maria Fenyö
, Alik Honigman
, Amos Panet
, J. Simon Gronowitz

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.

Original language	English
Pages (from-to)	1297-1303
Number of pages	7
Journal	AIDS Research and Human Retroviruses
Volume	6
Issue number	11
DOIs	https://doi.org/10.1089/aid.1990.6.1297
State	Published - Nov 1990

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title = "Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)",

abstract = "The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.",

author = "Torsten Unge and Harri Ahola and Ramagauri Bhikhabhai and Kristina B{\"a}ckbro and Seved L{\"o}vgren and Bror Strandberg and Feny{\"o}, \{Eva Maria\} and Alik Honigman and Amos Panet and Gronowitz, \{J. Simon\}",

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month = nov,

doi = "10.1089/aid.1990.6.1297",

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T1 - Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)

AU - Unge, Torsten

AU - Ahola, Harri

AU - Bhikhabhai, Ramagauri

AU - Bäckbro, Kristina

AU - Lövgren, Seved

AU - Strandberg, Bror

AU - Fenyö, Eva Maria

AU - Honigman, Alik

AU - Panet, Amos

AU - Gronowitz, J. Simon

PY - 1990/11

Y1 - 1990/11

N2 - The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.

AB - The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.

UR - https://www.scopus.com/pages/publications/0025224522

U2 - 10.1089/aid.1990.6.1297

DO - 10.1089/aid.1990.6.1297

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AN - SCOPUS:0025224522

SN - 0889-2229

VL - 6

SP - 1297

EP - 1303

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - 11

ER -

Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)

Abstract

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