TY - JOUR
T1 - Extinction of B-cell surface differentiation markers in hybrids between murine B-lymphoma and myeloma cells
AU - Laskov, Reuven
AU - Kim, K. Jin
AU - Kanellopoulos-Langevin, Colette
AU - Asofsky, Richard
PY - 1980
Y1 - 1980
N2 - Fusion between murine B-lymphoma cells bearing membrane IgM with either IgG or light chain secreting myeloma, resulted in cell hybrids synthesizing and secreting large quantities of IgM. In contrast, the hybrids did not secrete IgD even though it is also present on the surface of the B-lymphoma cells. B-Cell surface markers such as the IgM, IgD, Ia and the Fc receptor, which were present on the B-lymphoma cells, but not the myeloma cells were not expressed on the surface of the hybrids. Hybrids which secrete IgM and retain the B-cell membrane differentiation antigens were not detected, even when selection was done under conditions which favor the growth of the lymphoma parental cells.
AB - Fusion between murine B-lymphoma cells bearing membrane IgM with either IgG or light chain secreting myeloma, resulted in cell hybrids synthesizing and secreting large quantities of IgM. In contrast, the hybrids did not secrete IgD even though it is also present on the surface of the B-lymphoma cells. B-Cell surface markers such as the IgM, IgD, Ia and the Fc receptor, which were present on the B-lymphoma cells, but not the myeloma cells were not expressed on the surface of the hybrids. Hybrids which secrete IgM and retain the B-cell membrane differentiation antigens were not detected, even when selection was done under conditions which favor the growth of the lymphoma parental cells.
UR - http://www.scopus.com/inward/record.url?scp=0018935435&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(80)90158-6
DO - 10.1016/0008-8749(80)90158-6
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C2 - 6968630
AN - SCOPUS:0018935435
SN - 0008-8749
VL - 55
SP - 251
EP - 264
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -
