Abstract
The enzymatic activity of bone matrix vesicles from parathyroidectomized rats was determined and compared to the activity of vesicles from sham operated and normal animals. The vesicles were isolated from the alveolar bone by collagenase digestion and differential centrifugation and further purified on a discontinuous sucrose density gradient. The amount of extractable protein and the activity of alkaline phosphatase, acid phosphatase, and ATPase in the vesicle fractions thus obtained did not differ significantly from the values characteristic of preparations from control rats. It may therefore be suggested that parathyroid hormone depletion and the associated hypocalcemia have no significant effect on the occurrence and phosphatase activity of bone matrix vesicles.
Original language | English |
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Pages (from-to) | 451-457 |
Number of pages | 7 |
Journal | Cell and Tissue Research |
Volume | 228 |
Issue number | 3 |
DOIs | |
State | Published - Jan 1983 |
Keywords
- Bone matrix vesicles
- Mineralization
- Parathyroid hormone
- Rat