F-spondin promotes nerve precursor differentiation

David Schubert*, Avihu Klar, Minkyu Park, Richard Dargusch, Wolfgang H. Fischer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Cells in the developing nervous system secrete a large number of proteins that regulate the migration and differentiation of their neighbors. It is shown here that a clonal central nervous system cell line secretes a protein that causes both a rat hippocampal progenitor cell line and primary cortical neural cells to differentiate into cells with the morphological and biochemical features of neurons. This protein was identified as F-spondin. Analysis of F-spondin isoforms secreted from transfected cells shows that the core protein without the thrombospondin type 1 repeats is sufficient to promote neuronal differentiation when adsorbed to a surface. F-spondin can also inhibit neurite outgrowth while allowing the expression of nerve-specific proteins when present in a soluble form at high concentrations. Therefore, F-spondin can alter cell differentiation in multiple ways, depending upon its concentration and distribution between substrate-attached and soluble forms.

Original languageAmerican English
Pages (from-to)444-453
Number of pages10
JournalJournal of Neurochemistry
Volume96
Issue number2
DOIs
StatePublished - Jan 2006

Keywords

  • Differentiation
  • F-spondin
  • Nerve
  • Neural precursor

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