Fast-freezing devices for cryo-electron-microscopy

Shlomo Trachtenberg

doi:10.1016/0968-4328(93)90009-P

Fast-freezing devices for cryo-electron-microscopy

Shlomo Trachtenberg^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The design, construction and performance of two single fast-freezing devices are described. The devices are driven pneumatically at a controlled speed of up to 100msec^-1. They are suitable for either quench-freezing (in liquified gases) or for bounce-free impact-freezing on liquid nitrogen cooled metal blocks. Provisions are made to keep the metal block free of condensation and to allow multiple freezing.

Original language	English
Pages (from-to)	1-12
Number of pages	12
Journal	Micron
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/0968-4328(93)90009-P
State	Published - 1993

Keywords

Cryo-electron microscopy
fast-freezing
freeze substitution
freeze-drying
vitrification

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10.1016/0968-4328(93)90009-P

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abstract = "The design, construction and performance of two single fast-freezing devices are described. The devices are driven pneumatically at a controlled speed of up to 100msec-1. They are suitable for either quench-freezing (in liquified gases) or for bounce-free impact-freezing on liquid nitrogen cooled metal blocks. Provisions are made to keep the metal block free of condensation and to allow multiple freezing.",

keywords = "Cryo-electron microscopy, fast-freezing, freeze substitution, freeze-drying, vitrification",

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AB - The design, construction and performance of two single fast-freezing devices are described. The devices are driven pneumatically at a controlled speed of up to 100msec-1. They are suitable for either quench-freezing (in liquified gases) or for bounce-free impact-freezing on liquid nitrogen cooled metal blocks. Provisions are made to keep the metal block free of condensation and to allow multiple freezing.

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KW - fast-freezing

KW - freeze substitution

KW - freeze-drying

KW - vitrification

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Fast-freezing devices for cryo-electron-microscopy

Abstract

Keywords

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