Fate of compatible solutes during dilution stress in Ectothiorhodospira marismortui

Uri Fischel, Aharon Oren*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Ectothiorhodospira marismortui, a halophlic purple sulfur bacterium isolated from a sulfur spring on the shore of the Dead Sea, accumulates three organic solutes to achieve osmotic balance with the medium: glycine betaine, Nα-carbamoyl-l-glutamine 1-amide (CGA), and a minor amount of sucrose. When cells were subjected to dilution stress, part of the glycine betaine was excreted into the medium during the first 5-10 min, to be taken up again at a later stage. Intracellular concentrations of both CGA and sucrose decreased within 1-2 h to a new level corresponding with the lowered salinity. Neither CGA, nor sucrose appeared in the medium, showing that these compounds are metabolized intracellularly. When the decrease in intracellular CGA concentration was followed by assays for amide groups and for carbamoyl groups, it appeared that the decrease in carbamoyl groups containing material was much more rapid than the decrease in amide groups, suggesting l-glutamine 1-amide as a possible intermediate of CGA degradation.

Original languageAmerican English
Pages (from-to)113-118
Number of pages6
JournalFEMS Microbiology Letters
Volume113
Issue number1
DOIs
StatePublished - 1 Oct 1993

Bibliographical note

Funding Information:
We thank E.A. Galinski (Universityo f Bonn) for invaluabled iscussionsT. his work was sup-portedb y the G.I.F., the German-IsraeFlio unda-tion for Scientific Researcha nd Development, and by the BMFT-Minerva Gescllschaft fiir ForschungM, iinchen,G ermany.

Keywords

  • Ectothiorhodospira marismortui
  • Glycine betaine
  • N-carbamoyl-l-glutamine 1-amide
  • Osmoregulation
  • Sucrose

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