Fate of polyoma origin of replication after its direct introduction into mice

Hannah Cohen*, Nissim Benvenisty, Lea Reshef

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Recently we have developed a method for direct introduction of calcium phosphate-precipitated DNA into newborn rats. To examine whether the foreign DNA can replicate, a plasmid containing a polyoma origin of replication was injected into newborn mice. The plasmid was found intact in liver and spleen and able to transform bacteria. The foreign DNA had disappeared by the seventh day after injection. Yet, the plasmid DNA containing the polyoma origin of replication had undergone replication in both the liver and the spleen.

Original languageAmerican English
Pages (from-to)347-351
Number of pages5
JournalFEBS Letters
Issue number2
StatePublished - 2 Nov 1987

Bibliographical note

Funding Information:
We are indebted to Dr Oded Meyuhas for critical readingo f the manuscript.T his study was supported in part by grant 84-0016f7ro m the United States-Israel Binational Foundation (BSF), Jerusalem,I srael, and in part by a Wolfson grant from the Hebrew University, Jerusalem, Israel.


  • Polyoma
  • Replication
  • Transfection


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